Rye-cytoplasmic rj'e-wheat-additions were produced by double backcrossing rye-cytopiasmic tetraploid triticale w-ith an inbred line of dipioid rye cv. 'Esto'. Addition 6B was identified usmg LAP-
Wheat, rye and wheat-rye addition lines have been investigated regarding their developmental and organ-specific isozyme patterns of aspartate aminotransferase (AAT) and endopeptidase (EP). Evidence is given, that development-specific isozymes of AAT are encoded by chromosomes 3R and 4R of 'Imperial' rye which can be used as biochemical markers up to leaf age of 14 days. Organ-specific increase of intensity of bands for AAT in stems could be assigned to genes or alleies of chromosome 3A of 'Chinese Spring' wheat. For EP new markers were localized on chromosomes 4R and 6R of 'Imperial' rye showing variability. Utilization of these markers is possible at ail developmental stages of the leaves. Mechanisms of gene regulation are discussed.
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