PurposeTo determine the influence of age on central corneal thickness (CCT), endothelial cell density (ECD), average cell size, coefficient of variation in cell size, and percentage of regular hexagonal cells; and to estimate the average ECD and CCT in seven age groups.Materials and methodsAfter obtaining informed consent, 211 Caucasian patients (358 eyes) were examined using a noncontact specular microscope at the Center of Eye Diseases in Vilnius University Hospital Santariskiu Clinic. The main corneal parameters were: ECD, average cell size, coefficient of variation in cell size, percentage of regular hexagonal cells, and CCT. Subjects (20–89 years) were stratified by age into seven groups. Correlations between CCT, endothelial parameters (ECD, percentage of regular hexagonal cells, average, coefficient of variation), and age were found. Student’s t-test and Pearson’s correlation coefficient (r) values were calculated.ResultsA total of 114 (54.03%) women and 97 (45.97%) men participated in the study. Average ECD (cell/mm2) ranged from 2,931 (±371) in 20–29 year olds to 2,222 (±182) in 80–89 year olds; CCT (μm) ranged from 563 (±44) in 20–29 year olds to 540 (±35) in 80–89 year olds. A strong inverse correlation was observed between age and corneal ECD (r=−0.650, P<0.01) and a weak inverse correlation was observed between age and CCT (r=−0.156, P<0.01). ECD and CCT correlated directly (r=0.232, P<0.01). The average size of corneal endothelial cells directly correlated with age (r=0.586, P<0.01). There was no correlation between age and the coefficient of variation in cell size nor the percentage of regular hexagonal cells (P>0.05).ConclusionYoung people have higher ECD. CCT also decreases, but its dependence on age is weaker. A lower cell density indicates a thinner cornea. The variation in cell size and percentage of regular hexagonal cells are not dependent on age.

Diabetic patients have thicker corneas, lower ECD, and thinner subfoveal choroid than healthy subjects.

ABSTRACT.Purpose: To evaluate the changes of peripapillary and subfoveal choroidal thickness (CT) after trabeculectomy. Methods: Prospective longitudinal study included 37 eyes with open-angle glaucoma. The subfoveal and peripapillary CT was measured using enhanced depth imaging spectral domain optical coherence tomography before trabeculectomy and 1 week, 3 and 6 months postoperatively. The associations between changes in the CT, intraocular pressure (IOP) and axial length were analysed. Results: The medium subfoveal CT (IQR) increased from 182 (97) lm at baseline to 267 (107) lm 1 week, 213 (97) lm 3 months and 207 (91) lm 6 months postoperatively (p < 0.001). The peripapillary CT increased in all four quadrants at all follow-ups (p < 0.05). The subfoveal and peripapillary choroidal thickening correlated with the magnitude of IOP reduction (p < 0.05) and axial length shortening (p < 0.01) during whole follow-up period. There was a peripapillary CT increase of 2.9 lm per mmHg of IOP reduction (p < 0.001, CI 1.5-4.4) and 4.8 lm per mm of baseline axial length (p = 0.049, CI 0.03-9.6) 1 week postoperatively after adjustment for baseline IOP. Six months postoperatively, the decrease in axial length was the only factor associated with peripapillary choroidal thickening (p = 0.031; regression coefficient: 73.29 lm/mm, CI 7.1-139.5). Conclusion: Intraocular pressure (IOP) reduction after trabeculectomy caused the increase in subfoveal and peripapillary CT for at least 6 months postoperatively correlating with greater IOP reduction and axial length shortening. In the long term, the decrease in axial eye length, but not IOP, was the only factor to be associated with peripapillary choroidal thickening.

Amniotic membrane transplantation is an effective method of reconstruction following a conjunctival and limbal tumor excision and cryotherapy of surgical wound margins. In most cases, complete healing of an ocular surface can be achieved without any clinically significant complications.