Measurements are described of fusicoccin (FC)-stimulated H(+) efflux in barley (Hordeum vulgare L.) roots when K(+) and Na(+) concentrations were varied. In low-salt roots H(+) efflux was stimulated in both 5 mM KCl and NaCl. In salt-saturated roots H(+) efflux was stimulated more effectively in KCl than in NaCl solution. The stimulation of H(+) efflux thus is parallel with the selectivity of these different root preparations for K(+) and Na(+) and with estimates of permeability ratios (P Na/P K) determined from electrical measurements. It is suggested that the results support electrogenic coupling between FC-stimulated H(+) efflux and cation uptake.
Azetidine 2-carboxylic acid (AZ) was used as an analog of proline to investigate further the relationship between protein synthesis and ion transport. AZ does not inhibit protein assembly, but the proteins formed are ineffective as enzymes. At relatively low concentrations (50 5LM) AZ was a potent inhibitor of release of ions to the xylem of excised roots of barley (Hordeum vulgare L.) and intact plants. Uptake to the root was also inhibited but to a lesser degree. A procedure was introduced for estimating unidirectional fluxes from measurements of net tracer uptake, net transport to the xylem, and net efflux from the roots. It was shown that inhibition of release to the xylem was not caused by reduction in influx at the plasmalemma or to stimulation of influx to the vacuoles. It was suggested that AZ was acting on the process of release from symplast to the xylem. The action of AZ is compared with similar effects on ion transport produced by p-fluorophenylalanine, cycloheximide, and abscisic acid.
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