No abstract

No abstract

Point-of-interest (POI) recommendation is an important service to Location-Based Social Networks (LBSNs) that can benefit both users and businesses. In recent years, a number of POI recommender systems have been proposed, but there is still a lack of systematical comparison thereof. In this paper, we provide an all-around evaluation of 12 state-of-the-art POI recommendation models. From the evaluation, we obtain several important findings, based on which we can better understand and utilize POI recommendation models in various scenarios. We anticipate this work to provide readers with an overall picture of the cutting-edge research on POI recommendation.

A diverse group of cytolytic animal viruses encodes small, hydrophobic proteins to modify host cell membrane permeability to ions and small molecules during their infection cycles. In this study, we show that expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells. Immunofluorescent staining and cell fractionation studies demonstrate that this protein is an integral membrane protein. It is mainly localized to the ER and the Golgi apparatus. The protein can be translocated to the cell surface and is partially associated with lipid rafts. Further biochemical characterization of the protein reveals that it is posttranslationally modified by palmitoylation on all three cysteine residues. Systematic mutagenesis studies confirm that the membrane permeabilizing activity of the SARS-CoV E protein is associated with its transmembrane domain.

No abstract

The availability of user check-in data in large volume from the rapid growing location-based social networks (LBSNs) enables a number of important location-aware services. Point-of-interest (POI) recommendation is one of such services, which is to recommend POIs that users have not visited before. It has been observed that: (i) users tend to visit nearby places, and (ii) users tend to visit different places in different time slots, and in the same time slot, users tend to periodically visit the same places. For example, users usually visit a restaurant during lunch hours, and visit a pub at night. In this paper, we focus on the problem of time-aware POI recommendation, which aims at recommending a list of POIs for a user to visit at a given time. To exploit both geographical and temporal influences in time-aware POI recommendation, we propose the Geographical-Temporal influences Aware Graph (GTAG) to model check-in records, geographical influence and temporal influence. For effective and efficient recommendation based on GTAG, we develop a preference propagation algorithm named Breadth-first Preference Propagation (BPP). The algorithm follows a relaxed breathfirst search strategy, and returns recommendation results within at most 6 propagation steps. Our experimental results on two realworld datasets show that the proposed graph-based approach outperforms state-of-the-art POI recommendation methods substantially.

Lipid raft is an important element for the cellular entry of some viruses, including coronavirus infectious bronchitis virus (IBV). However, the exact role of lipid rafts in the cellular membrane during the entry of IBV into host cells is still unknown. In this study, we biochemically fractionated IBV-infected cells via sucrose density gradient centrifugation after depleting plasma membrane cholesterol with methyl-β-cyclodextrin or Mevastatin. Our results demonstrated that unlike IBV non-structural proteins, IBV structural proteins co-localized with lipid raft marker caveolin-1. Infectivity assay results of Vero cells illustrated that the drug-induced disruption of lipid rafts significantly suppressed IBV infection. Further studies revealed that lipid rafts were not required for IBV genome replication or virion release at later stages. However, the drug-mediated depletion of lipid rafts in Vero cells before IBV attachment significantly reduced the expression of viral structural proteins, suggesting that drug treatment impaired the attachment of IBV to the cell surface. Our results indicated that lipid rafts serve as attachment factors during the early stages of IBV infection, especially during the attachment stage.

Coronavirus envelope (E) protein is a small integral membrane protein with multi-functions in virion assembly, morphogenesis and virus-host interaction. Different coronavirus E proteins share striking similarities in biochemical properties and biological functions, but seem to adopt distinct membrane topology. In this report, we study the membrane topology of the SARS-CoV E protein by immunofluorescent staining of cells differentially permeabilized with detergents and proteinase K protection assay. It was revealed that both the N-and C-termini of the SARS-CoV E protein are exposed to the cytoplasmic side of the membranes (N cyto C cyto ). In contrast, parallel experiments showed that the E protein from infectious bronchitis virus (IBV) spanned the membranes once, with the N-terminus exposed luminally and the C-terminus exposed cytoplasmically (N exo(lum) -C cyto ). Intriguingly, a minor proportion of the SARS-CoV E protein was found to be modified by N-linked glycosylation on Asn 66 and inserted into the membranes once with the C-terminus exposed to the luminal side. The presence of two distinct membrane topologies of the SARS-CoV E protein may provide a useful clue to the pathogenesis of SARS-CoV.