Crop nitrogen is an efficient index for estimating crop yield. Using hyperspectral information to monitor nitrogen in cotton information in real time can help guide cotton cultivation. In this study, we used drip-irrigation cotton in Xinjiang as the research object and employed various nitrogen treatments to explore the correlation between hyperspectral vegetation indexes and leaf nitrogen concentration (LNC) and the canopy nitrogen density (CND) of cotton in different growth periods and interannual. We employed 30 published hyperspectral vegetation indexes obtained through spectral monitoring in 2019 and 2020 to screen for hyperspectral vegetation indexes highly correlated with the nitrogen in cotton indexes. Based on the same group of hyperspectral vegetation indexes, interannual and multi-temporal nitrogen estimation models of cotton were established using three modeling methods: simple multiple linear regression (MLR), partial least-squares regression (PLSR), and support vector regression (SVR). The results showed the following: (1) The correlations between LNC and CND and vegetation index in individual growth periods of cotton were lower than those for the entire growth period. The correlations between hyperspectral vegetation indexes and cotton LNC, CND, leaf area index (LAI), and aboveground biomass (AGB), were significantly different between years and varieties. The relatively stable indexes between vegetation and LNC were TCARI, PRI, CCRI, and SRI-2, and the absolute values of correlation were 0.251~0.387, 0.239~0.422, 0.245~0.387, and 0.357~0.533. In addition, the correlation between CIred-edge and REIlinear and group indicators (CND, AGB, and LAI) was more stable. (2) In the models established by MLR, PLSR, and SVR, the R2 value from the SVR method was higher in the estimation model based on the entire growth period data and LNC and CND. (3) Using the same group of selected hyperspectral vegetation indexes to estimate nitrogen in cotton in different growth stages, the accuracy of the estimation model of canopy nitrogen density (CND) was higher than that of the estimation model for leaf nitrogen concentration. The canopy nitrogen density most stable model was established by MLR at the flowering and boll stages and the full-boll stage with R2 = 0.532~0.665. This study explored the application potential of hyperspectral vegetation indexes to the nitrogen of drip-irrigated cotton, and the results provide a theoretical basis for hyperspectral monitoring for crop nutrients and canopy structure.

As an important index of a plant’s N nutrition, leaf nitrogen content (LNC) can be quickly monitored in real time with hyperspectral information, which is helpful to guide the precise application of N in cotton leaves. In this study, taking cotton dripping in Xinjiang, China, as the object of study, five N application treatments (0, 120, 240, 360, 480 kg·ha-1) were set up, and the hyperspectral data and the N content of main stem functional leaves at the cotton flower and boll stage were collected. The results showed that (1) comparing the correlations of the three types of spectral data from the original spectra, first derivative spectra, and second derivative spectra with the LNC of cotton, the first derivative spectra increased the correlation between the reflectance in the peak and valley ranges of the spectral curves and the LNC of cotton; (2) in the three hyperspectral regions of VIS, NIR, and SWIR, all R2 values of the estimation model for the LNC of cotton established based on the characteristic wavelengths of the original and the first derivative spectra were greater than 0.8, and the model accuracy was better than that of the second derivative spectra; and (3) the normalized root mean square error (n-RMSE) values of the validated model using MLR, PCR, and PLSR regression methods were all in the range of 10–20%, indicating that the established model could well estimate the nitrogen content of cotton leaves. The results of this study demonstrate the potential of the three hyperspectral domains of VIR, NIR, and SWIR to estimate the LNC of cotton and provide a new basis for hyperspectral data application in crop nutrient monitoring.

Potassium (K) is one of the most important elements influencing cotton metabolism, quality, and yield. Due to the characteristics of strong fluidity and fast redistribution of the K in plants, it leads to rapid transformation of the K lack or abundance in plant leaves; therefore, rapid and accurate estimation of potassium content in leaves (LKC, %) is a necessary prerequisite to solve the regulation of plant potassium. In this study, we concentrated on the LKC of cotton in different growth stages, an estimation model based on the combined characteristics of wavelet decomposition spectra and image was proposed, and discussed the potential of different combined features in accurate estimation of the LKC. We collected hyperspectral imaging data of 60 main-stem leaves at the budding, flowering, and boll setting stages of cotton, respectively. The original spectrum (R) is decomposed by continuous wavelet transform (CWT). The competitive adaptive reweighted sampling (CARS) and random frog (RF) algorithms combined with partial least squares regression (PLSR) model were used to determine the optimal decomposition scale and characteristic wavelengths at three growth stages. Based on the best “CWT spectra” model, the grayscale image databases were constructed, and the image features were extracted by using color moment and gray level co-occurrence matrix (GLCM). The results showed that the best decomposition scales of the three growth stages were CWT-1, 3, and 9. The best growth stage for estimating LKC in cotton was the boll setting stage, with the feature combination of “CWT-9 spectra + texture,” and its determination coefficients (R2val) and root mean squared error (RMSEval) values were 0.90 and 0.20. Compared with the single R model (R2val = 0.66, RMSEval = 0.34), the R2val increased by 0.24. Different from our hypothesis, the combined feature based on “CWT spectra + color + texture” cannot significantly improve the estimation accuracy of the model, it means that the performance of the estimation model established with more feature information is not correspondingly better. Moreover, the texture features contributed more to the improvement of model performance than color features did. These results provide a reference for rapid and non-destructive monitoring of the LKC in cotton.