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The combined use of multiple markers is considered a promising strategy in estimating the age of wounds. We sought to develop an "up, no change, or down" system and to explore how to combine and use various parameters. In total, 78 Sprague Dawley rats were divided randomly into a control group and contusion groups of 4-, 8-, 12-, 16-, 20-, 24-, 28-, 32-, 36-, 40-, 44-, and 48-h post-injury (n=6 per group). A contusion was produced in the right limb of the rats under diethyl ether anesthesia by a drop-ball technique; the animals were sacrificed at certain time points thereafter, using a lethal dose of pentobarbital. Levels of PUM2, TAB2, GJC1, and CHRNA1 mRNAs were detected in contused muscle using real-time PCR. An up, no change, or down system was developed with the relative quantities of the four mRNAs recorded as black, dark gray, or light gray boxes, representing up-, no change, or down-regulation of the gene of interest during wound repair. The four transcripts were combined and used as a marker cluster for color model analysis of each contusion group. Levels of PUM2, TAB2, and GJC1 mRNAs decreased, whereas that of CHRNA1 increased in wound repair (P<0.05). The up, no change, or down system was adequate to distinguish most time groups with the color model. Thus, the proposed up, no change, or down system provide the means to determine the minimal periods of early wounds.

This work studied a high winds‐producing bow echo that occurred over South China in the pre‐rainy season using radar observations and high‐resolution analyses from the Variational Doppler Radar Analysis System. The bow echo developed from a quasi‐linear convective system (QLCS) and acquired a well‐defined bow shape after merging with a pre‐line convective cell (CC). Interestingly, the rear‐inflow jet (RIJ), which has been well recognized to play a key role in the formation of a bow echo, was not found in this merger‐formation bow echo (MFBE). The reason is that the QLCS developed in the monsoon environment of high humidity and weak vertical wind shear, leading to a weak cold pool and line‐end vortices. A new pathway of bow echo formation was proposed which highlighted the importance of the low‐level mesovortex (MV) on the leading edge of the QLCS. Vertical vorticity budget analyses revealed that the MV originated from a weak vertical vorticity band ahead of the QLCS and grew rapidly during the merger, because of the enhanced low‐level convergence in between which led to vortexgenesis via vertical stretching of vertical vorticity. The up‐scale growth of the MV produced a RIJ‐like flow wrapping cyclonically from north of the QLCS which forced the system to bulge out and finally evolve into a bow echo. This MV contributed foremost to the near‐surface gales as well. These findings shed light on the diverse formation mechanisms of bow echoes and associated high winds in the moist monsoon environment of South China.

2020): Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats, Forensic Sciences Research, ABSTRACT In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n ¼ 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm 2 (0.5 mm  0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 mm 2 to 15 mm 2 , the FPR was 4.28%-12.14%, the FNR was 12.42%-64.08%, the sensitivity was 35.92%-87.58%, the specificity was 87.86%-95.72%, the Youden index was 0.316-0.754, accuracy was 82.80%-88.30%, and the AUC was 0.771-0.826. The AUC was largest when the cut-off value of the staining area was 12 mm 2 . Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases. ARTICLE HISTORY