A novel, sensitive, and specific competitive fluorescence immunoassay has been developed for the quantitative determination of dibutyl phthalate (DBP) using an antibody-coated plate format. Hapten was synthesized in order to produce polyclonal antibodies against dibutyl phthalate. Polyclonal antisera to dibutyl phthalate were generated in rabbits and used to construct the fluorescence immunoassay for measurement of dibutylphthalate. The assay had a detection limit of about 0.02 microg L(-1), a dynamic range of approximately 0.1-300 microg L(-1). Other similar phthalate compounds do not interfere significantly in the analysis using this immunoassay technique, and the cross-reactivity rates were less than 10%. The study demonstrated that the developed antiserum and fluorescence immunoassay procedure can be used to detect dibutyl phthalate in environmental samples such as tap water, river water, drinking water, and leachate from plastic drinking water bottles.
This study reported that the hapten of 2,4,6-trichlorophenol (2,4,6-TCP) was synthesized by using 2,4,6-TCP reacted with chloroactic acid in alkaline solution. The hapten was conjugated to bovine serum albumin (BSA) with the modified active ester method to form artificial immune antigen. The anti-TCP polyclonal antibodies were obtained by using the artificial immuneantigen (TCP-BSA) to immunize the rabbits. Using the purified antiserum of highest specificity, an antibody-coated fluoroimmunoassay was developed that shows an IC 50 of 4.8 mg/L with a limit of detection of 0.25 mg/L. The antibody showed negligible cross-reactivity with other phenols, which makes their assays suitable for the selective detection of 2,4,6-TCP. It shows a good accuracy and suitability to analyze, 2,4,6-TCP in environmental water.
The ability of phenols to quench the chemiluminescence of p-chlorobenzenediazonium fluoroborate in a medium of alkaline hydrogen peroxide was studied, and a flow injection chemiluminescent quench method for the determination of volatile phenols in polluted water was established. The linear calibration ranges are 0 4 . 0 , &5.0,0-4.5 and 0-8.0 jug ml-1 and the detection limits are 0.015, 0.020, 0.025 and 0.030 pg ml-1 for phenol, o-nitrophenol, p-cresol and 2,4-xylenol, respectively. This method is more sensitive than the 4-aminoantipyrine direct spectrophotometric method and the flow injection spectrophotometric method. The relative standard deviation is 3.0% for 1 pg ml-1 phenol.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.