Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.
The molecular structures
of polyamide barrier layers in reverse
osmosis membranes, made by interfacial polymerization of m-phenylenediamine and trimesoyl chloride under different reaction
and post-treatment conditions, were characterized by grazing incidence
wide-angle X-ray scattering (GIWAXS). The molecular backbone packing
is consistent with two different aromatic molecular packing motifs
(parallel and perpendicular) with preferential surface-induced orientation.
The results suggest that the perpendicular, T-shaped, packing motif
(5 Å spacing) might be associated with optimal membrane permeance,
compared with the parallel packings (3.5–4.0 Å spacings).
A 3-D surface shape measurement system based on a digital fringe projection and phase shifting technique is described. In this system, three phase-shifted fringe patterns and a centerline pattern are used to determine the absolute phase map of the object. This phase map is then converted to the x, y, and z coordinates of the object surface by a conversion algorithm. To determine the accurate values of the system parameters as required by the conversion algorithm, a two-step calibration procedure was developed. The parameters were first measured to determine their approximate values, then a calibration plate was measured by the system at various positions, and an iteration algorithm used to estimate the system parameters. Measurement results of several objects are presented. The standard deviation of the measurement error was found to be 0.23 mm.
This study revealed the effects of incorporating ionic liquid (IL) molecules: 1-ethyl, 1-butyl, and 1-octyl-3-methyl-imidazolium chlorides with different alkyl chain lengths, in interfacial polymerization (IP) on the structure and property (i.e., permeate-flux and salt rejection ratio) relationships of resulting RO membranes. The IL additive was added in the aqueous meta-phenylene diamine (MPD; 0.1% w/v) phase, which was subsequently reacted with trimesoyl chloride (TMC; 0.004% w/v) in the hexane phase to produce polyamide (PA) barrier layer. The structure of resulting free-standing PA thin films was characterized by grazing incidence wide-angle X-rays scattering (GIWAXS), which results were correlated with the performance of thin-film composite RO membranes having PA barrier layers prepared under the same IP conditions. Additionally, the membrane surface properties were characterized by zeta potential and water contact angle measurements. It was found that the membrane prepared by the longer chain IL molecule generally showed lower salt rejection ratio and higher permeation flux, possibly due to the inclusion of IL molecules in the PA scaffold. This hypothesis was supported by the GIWAXS results, where a self-assembled surfactant-like structure formed by IL with the longest aliphatic chain length was detected.
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