ESIPT-based fluorescence probes are emerging as an attractive tool for the detection of biologically relevant analytes owing to their unique photophysical properties. In this work, we have developed an ESIPT-based fluorescence probe (TCBT-OMe) for the detection of HClO/ClO- through the attachment of a bioorthogonal dimethylthiocarbamate linker. TCBT-OMe was shown to rapidly detect HClO/ClO- (<10 s) at biologically relevant concentrations (LoD = 0.16 nM) and have an excellent selectivity towards others ROS/RNS and amino acids. Therefore, TCBT-OMe was tested in live cells and was successfully shown to be able to detect endogenous and exogenous HClO/ClO- in HeLa cells. Additionally, TCBT-OMe acts as a dual input logic gate for Hg2+ and H2O2. Interestingly, Hg2+ alone gradually causes a fluorescence response but requires >30 min to produce a fluorescence response. Test strips containing TCBT-OMe were prepared and were demonstrated as an effective way to detect HClO/ClO- in water. Furthermore, TCBT-OMe was shown to detect exogenously added HClO/ClO- in three different water samples with little interference thus demonstrating the effectiveness as a method for the detection of HClO/ClO- in drinking water samples.
Ultrathin transition metal dichalcogenides (TMD) seem to have a promising future in the field of theranostic agent due to their excellent near-infrared light absorption capacity and large specific surface area....
The
ultrasmall sizes of nanoparticles have attracted significant
attention for potential applications in the fields of catalysis and
nanomedicine. Herein, we reported on the green preparation and X-ray
computed tomography (CT) imaging of ultrasmall bimetallic bovine serum
albumin-directed gold–silver (Au–Ag@BSA) nanoparticles
(2–4 nm) using BSA as a stabilizing and template-directed agent.
Further, the effects of synthesis conditions were systematically explored
to prepare products by adjusting the different molar ratios of Au/Ag.
The resulting Au–Ag@BSA nanoparticles exhibited the spherical
shape, well-dispersed ability, as well as long-term room-temperature
stability. The cytotoxicity effects of Au–Ag@BSA nanoparticles
on A549 and MCF-7 cells were compared with those of individual Ag
nanoparticles, and the results indicated a lower cytotoxicity effect
by Au–Ag@BSA nanoparticles. Furthermore, the in vivo toxicity of Au–Ag@BSA nanoparticles was investigated in the
early stage zebrafish embryos. The results indicate that there are
not any obvious changes of survival and hatching percentages at multiple
growth stages (4–120 hpf) even with a high level of Au–Ag@BSA
nanoparticles (up to 80 mM), revealing good biocompatibility. Interestingly,
a rational design of the Au/Ag molar ratio (3:2) surprisingly possessed
enhanced CT performance compared to the performance of the Au nanoparticles
and iohexol. Accordingly, this study highlights a new prospect in
the green preparation of ultrasmall alloy nanomaterials with good
biocompatibility and will be of great interest in developing CT contrast
agent, catalyst, as well as drug delivery carrier.
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