Summary
Emerging evidences have suggested that liquid–liquid phase separation (LLPS) of proteins plays a vital role both in a wide range of biological processes and in related diseases. Whether a protein undergoes phase separation not only is determined by the chemical and physical properties of biomolecule themselves, but also is regulated by environmental conditions such as temperature, ionic strength, pH, as well as volume excluded by other macromolecules. A web accessible database LLPSDB was developed recently by our group, in which all the proteins involved in LLPS in vitro as well as corresponding experimental conditions were curated comprehensively from published literatures. With the rapid increase of investigations in biomolecular LLPS and growing popularity of LLPSDB, we updated the database, and developed a new version LLPSDB v2.0. In comparison of the previously released version, more than double contents of data are curated, and a new class “Ambiguous system” is added. In addition, the web interface is improved, such as that users can search the database by selecting option “phase separation status” alone or combined with other options. We anticipate that this updated database will serve as a more comprehensive and helpful resource for users.
Availability and implementation
LLPSDB v2.0 is freely available at: http://bio-comp.org.cn/llpsdbv2
Supplementary information
Supplementary data are available at Bioinformatics online.
To give cellulose fibers dual characteristics of warming and fluorescence, graphene oxide (GO) and fluorescent particles were simultaneously dispersed into the regenerated cellulose spinning solution through blending modification and post-reduction methods. After dry-jet wet spinning and reducing in hydrazine hydrate solution, the reduced graphene oxide (RGO)/regenerated composite fibers with different mass ratios of filler were completed. Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), fluorescence microscopy, and other methods were used to characterize the structure and morphology of fibers. Test results showed that the thermal stability and infrared emissivity increased gradually with the increase of GO. The crystallinity and strength of the composite fiber first increased and then decreased. This type of fiber also had luminescent properties after addition of fluorescent agent. However, when too much fluorescent agent was added, the thermal stability, infrared emissivity, crystallinity, and other properties mentioned above were affected to some extent. According to the comprehensive analysis, when the amount of GO added was 1 wt% and fluorescent added was 3 wt%, respectively, the luminescence characteristic and far-infrared emissivity of the fibers were remarkably improved.
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