The use of competitive ratiometric fluorescence indicator displacement chemosensors derived from two alkyl substituted cucurbit[6]uril‐based host‐guest complexes is reported. In particular, the differing binding abilities of two cucurbit[6]uril derivatives towards the target analytes led to a useful ratiometric detection signal output for the discrimination of lysine and methionine versus the other tested α‐amino acids in aqueous solution.
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