Background and Aims Hepatocellular carcinoma (HCC) is a common malignant disease with high morbidity and mortality throughout the world. While Borealin is a putative oncogene that is dysregulated in multiple tumors, its exact role in HCC remains less investigated. Methods Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) assays were employed to examine the relative amount of Borealin. Gene set enrichment analysis (GSEA) and other bioinformatic analyses were implemented to probe into the potential functions of Borealin. The biological roles and mechanisms of Borealin in the tumorigenesis and development of HCC were further evaluated using a battery of functional assays in vivo and in vitro. Results Borealin was enhanced in the HCC tissue samples and hepatoma cells when compared with the nontumor tissues and normal liver cells. Higher Borealin expression was positively linked with advanced pathological phenotypes and inferior overall survival. The overexpression of Borealin promoted the cells’ abilities on proliferation, invasion and epithelial–mesenchymal transition (EMT) in vitro, facilitated tumor growth and lung metastasis in vivo, whereas the silencing of Borealin inhibited these capabilities in vitro. Furthermore, Borealin interacted with β-catenin and further activated the Wnt/β-catenin signaling pathway, which endowed HCC cells with highly aggressive and metastatic capabilities. Conclusion Borealin was identified as an oncogene that could promote HCC growth and metastasis by activating the WNT/β-catenin signaling pathway. These findings extended the understanding of Borealin in HCC tumorigenesis and development and highlighted the significance of Borealin in HCC diagnosis and treatment.
A Modular Reconfigurable Robot (MRR) composed of standard joint and link modules has the advantages of rapid changeover of assembly configurations for a diversity of application requirements. To tackle the kinematics analysis issues for an MRR, it is essential to develop a generic method to mathematically represent all possible assembly configurations and automatically generate their kinematic models. In this paper, two types of robot modules, i.e., revolute joint modules and rigid link modules, are considered as the basic building blocks of an MRR. The topological structure of an MRR is represented by a graph, termed an Assembly Graph (AG), in which all robot modules, regardless of their types, are treated as vertices, while the connecting interfaces between any two robot modules are treated as edges. Based on graph theory, a modified adjacency matrix, termed an Assembly Adjacency Matrix (AAM), is proposed to represent the assembly configuration of an MRR, in which the three-dimensional assembly information is specified with connecting port vectors for the adjacent modules. A path matrix derived from the AAM of an MRR is employed to describe the connecting sequence of its constituting modules. The local frame representation of the Product-of-Exponentials (POE) formula is employed, which is configuration-independent. Therefore, for an MRR configuration represented with an AAM, its kinematic model will be automatically generated according to its path matrix. The proposed assembly configuration representation and kinematic modeling method are validated through a dual-branch MRR configuration.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.