Background Pyroptosis plays a dual role in the development of cancer and malignancy, and as such, may potentially be a new target for cancer treatment. However, the inflammatory response to pyroptosis may have adverse effects on the body. The roles of gasdermin E (GSDME), caspases, and related proteins associated with pyroptosis in cancer remain controversial. This study aimed to explore whether the expression levels of caspase-3 and GSDME affect the clinical stage, pathological grade, and survival prognosis of patients with lung cancer. Methods We examined the protein levels of GSDME, caspase-3, caspase-8, and caspase-9 in lung tissues from 100 patients with lung cancer by using immunohistochemistry. Results We found that GSDME, caspase-3, and caspase-8 were more highly expressed in the tumor tissues than in the adjacent normal tissues. Moreover, we found that GSDME could serve as a prognostic factor because there was a positive correlation between its expression level and the postoperative survival rate of patients with lung cancer. Conclusions GSDME may be an independent factor affecting the prognosis of patients with lung cancer. However, the role of GSDME and its related proteins in cancer requires further research.
Objectives Endometrial carcinoma originates from the endometrium and is one of the most common gynecological malignancies worldwide. With appropriate treatment, the 5-year survival rate for endometrial carcinoma is approximately 80%. Therefore, recent studies have focused on finding tumor-specific markers that can predict the biological behavior of endometrial carcinoma in relation to cell motility and invasion. Methods The expression of p27kip1 gene between 130 endometrial carcinoma tissues and normal endometrial tissues were analyzed through immunohistochemistry. The endometrial carcinoma cell lines Ishikawa(ISK) were cultured, the p27kip1-saRNA expression vector was constructed, and the endometrial carcinoma cells interfering with the expression of p27kip1 gene was also established. Western blotting and RT-PCR were employed to verify whether saRNA could up-regulate the expression of p27kip1 gene. Additionally, the proliferation, migration and invasion capacities in cell line after activating with p27kip1 was detected through CCK-8 and Transwell assay respectively. Furthermore, MMP2 and MMP9 were detected by ELISA and epithelial-mesenchymal transition (EMT) related proteins including E-cadherin, N-cadherin and Vimentin were assessed by Western blotting analysis. Results Compared with normal endometrial tissues, the p27kip1 expression levels in endometrial carcinoma tissues were decreased remarkably. saRNAs transfection significantly up-regulated expressions in endometrial carcinoma cell lines. The proliferation, invasion and migration ability were inhibited considerably by p27kip1-saRNA compared with the control group. The expression of E-cadherin was increased while the expression of N-cadherin, Vimentin, MMP-2 and MMP-9 was decreased activated by the p27kip1-saRNA. Conclusions p27kip1 could be used as a prognostic factor of endometrial carcinoma. Activating the expression of p27kip1 in endometrial carcinoma cell line could effectively inhibit the proliferation, invasion and migration, and its mechanism is related to the inhibition of EMT. p27kip1 is potential target for the treatment of endometrial carcinoma.
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