Summary -Hemoglobin ( Hb ) could be used as a substitute of peroxidase in the catalytic oxidation of tetra-substituted amino aluminum phthalocyanine ( TAAlPc ) by H 2 O 2 . We found that the fluorescence of TAAlPc (a red-region fluorescent dye with a maximum excitation wavelength at 606 nm and a maximum emission wavelength at 673 nm) could significantly be quenched by H 2 O 2 in the presence of Hb. The value of F 0 /F ( where the relative fluorescence intensity of blank solution and that of the sample solution containing Hb were given by F 0 and F, respectively) is linearly related to the concentration of Hb. Based on this, a novel fluorimetric method was developed for the determination of Hb in aqueous solution. Under optimal conditions, Hb could be determined in the concentration range of 5× 10 -11 -1.2×10-8 mol L -1 with a detection limit of 1.5×10 -11 mol L -1 . The relative standard deviation of ten replicate measurements was 1.95% for solution containing 1×10 -9 mol L -1 Hb. The proposed method has been applied to the analysis of Hb in human blood and the results were in good agreement with those reported by a hospital laboratory. So this is a new, high sensitive and precise fluorescence quenching method to determine Hb.
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