Phosphorylation is one of the most important protein post‐translational modifications, which possesses dramatic regulatory effects on the function of proteins. In consideration of the low abundance and low stoichiometry of phosphorylation and non‐specific signal suppression, efficient capture of the phosphoproteins from complex biological samples is critical to meet the need for protein profiling. In this work, a facile preparation of titanium (IV)‐immobilized O‐phospho‐L‐tyrosine modified magnetic nanoparticles was developed for the enrichment of intact phosphoproteins. The prepared magnetic nanoparticles were characterized by various instruments and had a spherical shape with an average diameter of 300 nm. The adsorption isotherms were investigated and the maximum capacity for β‐casein was calculated to be 961.5 mg/g. Standard protein mixtures and biological samples (non‐fat milk and human serum) were selected to test the enrichment performance. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis demonstrated the excellent enrichment performance with high selectivity. With the superparamagnetic property, titanium (IV)‐immobilized O‐phospho‐L‐tyrosine modified magnetic nanoparticles were convenient for the practical application and clinical promotion, thus having a promising prospect in the field of phosphoprotein research.
The influence of support parameters on the parametric resonance of fluid conveying pipeline under pulsating flow excitation is studied. Firstly, the mathematical model of fluid conveying pipeline and support system is established, Then the Galerkin method is used for discretization, and the incremental harmonic balance method is used to solve the problem. The effects of support stiffness, support damping, support position, support number and support spacing on the boundary of parametric resonance region and the parametric resonance response are discussed. The results show that under the fixed pulsating amplitude, the pulsating frequency range of system instability becomes smaller with the increase of support stiffness, support damping and the number of support, while the support position and support spacing have less influence on it. The change of support stiffness and support position has little influence on the pulsation frequency that the parametric resonance lasts to the end, but the increase of brace damping makes its value decrease obviously. The research results can provide a theoretical basis for the design and installation of pipeline support system.
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