Bone mesenchymal stem cells (BMSCs) transplantation is a promising therapeutic approach for myocardial infarction (MI), but its application is limited by poor viability of BMSCs. In this study, we aimed to improve the survival of BMSCs by lentivirus vector mediated overexpression of integrin β1. In vitro study showed that integrin β1 overexpression could facilitate the proliferation of BMSCs under oxygen glucose deprivation condition and regulated the expression of Caspase-3, Bax, Bcl-2, FAK, and ILK in BMSCs. Next, MI was induced in rat model and Igtb1BMSCs, NullBMSCs, or NatBMSCs were transplanted by intramyocardial injection. One week later, the survival of BMSCs was higher in Itgb1 BMSCs group than in other groups. Four weeks after transplantation, heart function was significantly improved in Igtb1BMSCs group compared to other groups. The expression levels of Caspase-3 and Bax were decreased while the expression levels of Bcl-2, FAK, ILK, and VEGF were increased in the cardiomyocytes of Igtb1BMSCs group compared to other groups. In conclusion, integrin β1 overexpression could increase the survival of BMSCs and improve the efficacy of transplanted BMSCs for MI treatment. The beneficial effects may be mediated by inhibiting the apoptosis of both transplanted BMSCs and cardiomyocytes through adhesion-mediated cell survival signaling.
The palladium-catalyzed selective carbofunctionalization of inert γ-C(sp 3)À O bonds of aliphatic amides by using the strategy of sp 3 CÀ H activation/β-O elimination/hydrocarbofunctionalization with the assistance of 8-aminoquinolyl directing group is presented here. This method features a broad scope of both 4-hydroxypyridin-2(1H)-ones and 4-hydroxy-2H-pyran-2-ones, providing a versatile method for the synthesis of corresponding γsubstituted amide derivatives.
Background:
Metabolic syndrome (MS) is showing an epidemic trend worldwide, and its annual incidence is increasing. Conventional treatment options have limitations and it is necessary to develop new management strategies for MS.
Methods:
Eligible randomized controlled clinical trials (RCTs) were screened by searching multiple Chinese and English databases. References to the included literature, gray literature in OpenGrey, and other relevant literature, such as clinical studies registered in ClinicalTrials.gov, were also manually searched. Relevant data were extracted, and meta-analysis was performed using Reviewer Manager 5.4.
Results:
This study provides a high-quality review of the efficacy and safety of acupuncture in the treatment of MS, and provides a basis for the clinical application of acupuncture in the treatment of MS.
Conclusion:
This study provides evidence of the effectiveness and safety of acupuncture in the treatment of MS.
The inside cover picture shows the palladium‐catalyzed selective carbofunctionalization of inert γ‐C(sp3)‐O bonds of aliphatic amides by using the strategy of sp3 C‐H activation/β‐O elimination/hydrocarbofunctionalization with the assistance of 8‐aminoquinolyl directing group. Notable features of the strategy include (i) the use of hydroxypyridones and analogous hydroxypyrones as nucleophilic reagents for the first time to achieve carbofunctionalization of C(sp3)‐O bonds, (ii) the realization of inert γ‐methyl and methylene C(sp3)‐O bonds cleavage, and (iii) high functional group tolerance. More information can be found in the Update by Lin et al. The cover artwork was composed by Cong Lin and Liang Shen. (Q. Guan, C. Lin, S. Chen, F. Gao, L. Shen, Adv. Synth. Catal. 2020, 362, 5772–5776; DOI: 10.1002/adsc.202001091).
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