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Zhang et al. R. intraradices Alleviates Arsenic Toxicity in R. pseudoacacia altering root morphology, regulating the concentrations and ratios of phytohormones, and increasing the concentration of soil glomalin. The results suggested that AMFinoculated R. pseudoacacia seedlings would be a critical factor in successful vegetation restoration and soil development in As-contaminated soils.

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Purpose Arbuscular mycorrhizal fungi (AMF) are capable of protecting host plants against heavy metal toxicity, whereas rare knowledge has been acquired on the underlying effects of AMF on woody leguminous species under arsenic (As) stress. This study was aimed that whether AMF inoculation improves the As tolerance in Sophora viciifolia (a king of leguminous shrub). Methods S. viciifolia seedlings were inoculated with AMF Rhizophagus intraradices, and then the seedlings were grown at three levels (0, 50, and 100 mg As kg−1 soil) of As-polluted soil by performing the potted experiments. The plant growth, photosynthetic parameter, oxidative damage, antioxidant enzyme activities and gene expression of phytochelatin synthase in R. intraradices- and non-inoculated S. davidii seedlings under the different levels of As stress were analyzed. Result Compared with non-inoculated S. viciifolia at the identical As level, R. intraradices-inoculated S. viciifolia were higher in the shoot and root dry weight, plant height, root length, photosynthetic rate, stomatal conductance, transpiration rate, maximal photochemical efficiency of PSII photochemistry, actual quantum yield, and photochemical quenching values, while the intercellular CO2 concentration and non-photochemical quenching values were lower. As-induced oxidative stress generating malondialdehyde, hydrogen peroxide and superoxide in the S. viciifolia leaves and roots reduced significantly by R. intraradices inoculation, whereas the activities of antioxidative enzymes (e.g., superoxide dismutase, peroxidase, and catalase) in S. viciifolia leaves and roots were increased by R. intraradices inoculation. Notably, R. intraradices inoculation up-regulated the gene expression of S. viciifolia phytochelatins in the leaves and roots. Conclusion These results demonstrated that R. intraradices inoculation enhanced the As tolerance in S. viciifolia seedlings by improving the plant growth, gas exchange, chlorophyll fluorescence, reactive oxygen species, antioxidant enzymes and gene expression of S. viciifolia phytochelatins. The present study verified a multifarious positive role of AMF for woody leguminous species under As stress.

Nitrogen (N) is an essential nutrient that plants require and is, most of the time, limited in different terrestrial ecosystems. Forming symbioses with plants, arbuscular mycorrhizal (AM) fungi improve mineral element uptake and the net primary production of plants. Recent reports have suggested that AM fungi mediate N uptake in plants. However, there are fewer studies on the influence of AM fungi on the response of Lycium barbarum, a medicinal plant in northwest China, under different N-addition conditions. In this study, the effect of Rhizophagus irregularis, N forms (NO3− and NH4+), and N levels (1.5, 7.5, 15, 30 mM) on the performance of L. barbarum was evaluated through a pot experiment. The application of R. irregularis significantly improved L. barbarum biomass, net photosynthetic rate, and root tissue viability under adequate NO3− and NH4+ supplies, and mycorrhizal plants showed better performance under NO3− supply. AM colonization enhanced N acquisition under adequate NO3− supply and strongly induced the expression of LbAMT3-1 in L. barbarum roots. Based on these results, we propose that NO3−-dominated N supply favors mycorrhizal symbiosis to a greater extent than NH4+; this study provides a basis for maintaining beneficial AM symbiosis during nitrogen fertilizer use in arable land.