The
stimuli-responsive DNA hydrogel has attracted wide attention
in the fields of chemical and biological sensing. However, it is still
a challenge to integrate characteristics with low-cost, high mechanical
strength, and signal self-expression into a DNA hydrogel simultaneously.
Herein, a stimuli-responsive 2D photonic crystal double network DNA
hydrogel (2D PhC DN-DNA hydrogel) sensing platform is developed via
combining the signal self-expression of 2D PhC array with the selective
recognition of polyacrylamide (PAM)/DNA DN hydrogel. The change of
DNA configuration induced by specific target triggers the change of
2D PhC DN-DNA hydrogel volume, leading to a shift of the Debye diffraction
ring diameter. In order to verify the feasibility of this strategy,
the 2D PhC DN-DNA hydrogel with C-rich sequences is chosen as a proof-of-concept.
The results indicate that the hydrogel has good detection performance
for pH and Ag+/Cys. And the Debye diffraction ring diameter
of the hydrogel is correlated with the concentration of the Ag+/Cys in the range of 0.5–20 μM. Compared with
previously pure DNA hydrogel sensing platform, the 2D PhC DN-DNA hydrogel
features low-cost preparation process and label-free determination.
Meanwhile, only a laser pointer and a ruler are needed for the determination
of targets, which shows that the hydrogel has application prospect
in the development of portable response equipment.
A simple penicillinase functionalized two-dimensional photonic crystal hydrogel (2DPPCH) biosensor was developed for colorimetric detection of penicillin G and penicillinase inhibitor. The penicillinase can specifically recognize penicillin G and catalyze...
A simple, label-free, and visual photonic crystal-based β-lactamase biosensor was developed for β-lactam antibiotic and β-lactamase inhibitor in which the penicillinase (a β-lactamase) was immobilized on the pH-sensitive colloidal crystal hydrogel (CCH) film to form penicillinase colloidal crystal hydrogel (PCCH) biosensing film. The hydrolysis of penicillin G (a β-lactam antibiotic) can be catalyzed by penicillinase to produce penicilloic acid, leading to a pH decrease in the microenvironment of PCCH film, which causes the shrink of pH-sensitive CCH film and triggers a blue-shift of the diffraction wavelength. Upon the addition of β-lactamase inhibitor, the hydrolysis reaction is suppressed and no clear blue-shift is observed. The concentrations of β-lactam antibiotic and β-lactamase inhibitor can be sensitively evaluated by measuring the diffraction shifts. The minimum detectable concentrations for penicillin G and clavulanate potassium (a β-lactamase inhibitor) can reach 1 and 0.1 μM, respectively. Furthermore, the proposed method is highly reversible and selective, and it allows determination of penicillin G in fish pond water samples.
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