Changjiang (Yangtze River) Estuary has experienced severe hypoxia since the 1950s. In order to investigate potential ecological functions of key microorganisms in relation to hypoxia, we performed 16S rRNA‐based Illumina Miseq sequencing to explore the bacterial diversity in the surface sediments of the hypoxic zone near the Changjiang Estuary and in the East China Sea (ECS). The results showed that numerous Proteobacteria‐affiliated sequences in the sediments of the inner continental shelf were related to both sulfate‐reducing and sulfur‐oxidizing bacteria, suggesting an active sulfur cycle in this area. Many sequences retrieved from the hypoxic zone were also related to Planctomycetes from two marine upwelling systems, which may be involved in the initial breakdown of sulfated heteropolysaccharides. Bacteroidetes, which is expected to degrade high‐molecular‐weight organic matter, was abundant in all the studied stations except for station A8, which was the deepest and possessed the largest grain size. In addition, dissolved organic carbon, water depth, percentage ratio of clay to silt, salinity, and sedimentary grain size were environmental effectors that shaped the sedimentary microbial community structure. Our results showed that putative Gammaproteobacteria‐affiliated sulfur‐oxidizing bacteria may not only detoxify hydrogen sulfide produced by sulfate‐reducing prokaryotes, but also serve as the primary producers in the marine sediments. Specific groups of aerobic Bacteroidetes and Planctomycetes participated in degrading organic matter, which might contribute to the oxygen depletion in the hypoxic zones.
Five new sesquiterpene derivatives, including dihydroagarofuran pyridine macrolides 1-4 and dihydroagarofuran ester 18, and 13 known dihydroagarofuran derivatives were isolated from the aqueous EtOH extract of the dried roots of Tripterygium wilfordii. An in vitro antiherpetic activity assay indicated that compounds 11 and 17 displayed weak and moderate inhibition against herpes simplex virus type II, respectively.
Thunder god vine, the dried roots of Tripterygium wilfordii, is a widely used traditional Chinese medicine. More than 200 bioactive complex natural products have been isolated from this herb. Inspired by the diversity of chemical structures and bioactivities of the components of this herb, the investigation to mine new chemical entities as potential drug leads led to the identification of 36 nitrogen-containing compounds. Among them, 18 new dihydro-β-agarofuran alkaloids (tripterygiumines A-L (1-12), M-Q (22-26), and R (33)) were identified from the spectroscopic data and chemical degradation studies. Tripterygiumine Q (26) exhibited immunosuppressive activity against human peripheral mononuclear cells with an IC50 value of 8.67 μM and showed no cytotoxicity, even at 100 μM, indicating that 26 may represent a novel scaffold for the development of new immunosuppressants.
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