A high-sensitivity ultrasonic sensing system is proposed and demonstrated. In this system, a phase-shifted fiber Bragg grating (PS-FBG) is used as a sensor to achieve broadband and highly sensitive detection. The PS-FBG modulates the output of a tunable laser to detect the ultrasonic strain directly. Balanced photo-detector (BPD) is used for eliminating the DC component and further amplifying the AC component in the detected signal. Another major function of the BPD is to reject laser intensity noise. As a result, the minimum detectable strain is limited by the BPD's noise and laser frequency noise. The sensitivity of the system is 9 nε/Hz(1/2). Because of its high sensitivity, this system has the potential to be used in acousto-ultrasonic testing without amplifying the input signal and in practical acoustic emission detection.

We report on a resonant waveguide grating imager for high throughput screening using live cells. This imager can generate a snapshot image of all biosensors in a 384-well microtiter plate with a time resolution of ∼3 s and a spatial resolution of 80 μm. This imager is well tolerant to variability in plate configurations and cell confluency. The resonant wavelength and its shifts induced by cell responses at each pixel correlate well with cell confluency. Data filtration protocol can be used to improve assay quality for partially confluent cells.

The β2-adrenergic receptor (β2AR) agonists with reduced tachyphylaxis may offer new therapeutic agents with improved tolerance profile. However, receptor desensitization assays are often inferred at the single signaling molecule level, thus ligand-directed desensitization is poorly understood. Here we report a label-free biosensor whole cell assay with microfluidics to determine ligand-directed desensitization of the β2AR. Together with mechanistic deconvolution using small molecule inhibitors, the receptor desensitization and resensitization patterns under the short-term agonist exposure manifested the long-acting agonism of salmeterol, and differentiated the mechanisms of agonist-directed desensitization between a full agonist epinephrine and a partial agonist pindolol. This study reveals the cellular mechanisms of agonist-selective β2AR desensitization at the whole cell level.

The fiber Bragg grating (FBG) sensor, which was developed over recent decades, has been widely used to measure manifold static measurands in a variety of industrial sectors. Multiple experiments have demonstrated its ability in ultrasonic detection and its potential in ultrasonic structural health monitoring. Unlike static measurements, ultrasonic detection requires a higher sensitivity and broader bandwidth to ensure the fidelity of the ultrasonic Lamb wave that propagates in a plate-like structure for the subsequent waveform analysis. Thus, the FBG sensor head and its corresponding demodulation system need to be carefully designed, and other practical issues, such as the installation methods and data process methods, should also be properly addressed. In this review, the mature techniques of FBG-based ultrasonic sensors and their practical applications in ultrasonic structural health monitoring are discussed. In addition, state-of-the-art techniques are introduced to fully present the current developments.