Non-Tuberculous Mycobacteria (NTM) have frequently been isolated from metalworking fluids (MWFs) used in large machining industries. This paper concerns the microbial detection, particularly NTM in MWFs employed in small metalworking shops. A total of 21 samples were collected from different turneries and were tested for several microbiological parameters. A total microbial count above 10 6 CFU ml -1 was observed in 66.6% (14/21) of samples and none of the samples had the count <10 2 CFU ml -1 . The dominant contaminants were Gram-negative bacteria with 90.5% (19/21) of samples revealing the presence of Pseudomonas aeruginosa. Sulfate Reducing Bacteria (SRB) were detected in 52.4% (11/21) of samples, whereas NTM were recovered from 14.3% (3/21) of samples only. Two species of NTM were identified by biochemical reactions as Mycobacterium chelonae and Mycobacterium abscessus. It was concluded that MWFs used in small turneries are usually contaminated with Gram-negative bacteria and SRB but NTM are not common contaminants of these fluids.
"Objectives: Sulphate Reducing Bacteria (SRB) are part of the normal oral microbiota. They have been frequently detected in the oral cavity of individuals with and without oral manifestations. The aim of this investigation was to determine possible correlation between SRB count and their activity in saliva versus some oral health manifestations.
Methods: A total of 64 saliva samples were collected from healthy individuals, and patients with several oral manifestations. Detection and count of SRB were performed using documented methods, while sulphide ion concentration which is the metabolic end product of these bacteria was measured using a silver electrode connected to a millivolt meter.
Results: SRB were detected in 35.9% of saliva samples investigated. Statistical significance was observed between the detection of these bacteria and various oral conditions. However, count of SRB and the sulphide ion concentration in the tested samples did not have significant correlation with the oral conditions considered.
Conclusion: The mere detection of SRB in saliva is of significance in the diagnoses of oral conditions. Enumeration of these bacteria and quantification of their metabolic end product in the same samples were not of diagnostic value."
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