Hybridisation was carried out between stenospermocarpic seedless grape cultivars (female parents) and Chinese wild Vitis amurensis or its hybrids with cold-resistance alleles (male parents). The aim of this paper was: (1) to evaluate the effects of the addition of the plant growth regulator indole-3-acetic acid (IAA) to the embryo development medium MM3 + 500 mg L −1 casein hydrolysate + 1 mmol L −1 serine; (2) to obtain a batch of seedless hybrid grape offspring by embryo rescue; and (3) to screen suitable genotypes for embryo rescue. The results indicated that adding 1.75 mg L −1