The objective of the present study is to evaluate the anisotropy influence on the mechanical and microstructural characteristics of AZ31B magnesium alloy sheets deformed at room and elevated temperature. To this aim, uniaxial tensile tests were carried out along the rolling, transverse and diagonal direction in the range of temperatures from room temperature to 300°C at 0.1 s-1. The Lankford coefficients, ultimate tensile strength, diffuse necking strain and fracture strain values were evaluated as a function of the testing temperature and specimen orientation. Furthermore, microstructural features were analysed as well as micro-hardness was measured for each testing condition to assess the post-deformation characteristics.