The study was conducted to evaluate the effects of olive leaves with probiotics (OLP) on growth performance, carcass characteristics, meat quality, blood parameters and oxidative stability of meat in broiler. A total of 160 day old, unsexed Cobb-500 chicks were distributed in to five dietary treatment groups: Control (Basal diet), OLP-1 (Basal diet + 0.4% OLP, DM basis), OLP-2 (Basal diet + 0.8% OLP, DM basis), OLP-3 (Basal diet + 1.2% OLP, DM basis) and OLP-4 (Basal diet + 1.6% OLP, DM basis) having 4 replications with 8 birds in each in a completely randomized design. Results showed that the live weight, overall average daily gain (ADG) increased significantly (p<0.05) in all treatment groups compared to control. The weekly feed conversion ratio (FCR) reduced significantly (p<0.05) in 0-14 days and the weekly ADG improved in 15-28 days. Blood cholesterol and high density lipoprotein (HDL) increased significantly (p<0.05) in all treatment groups except OLP-4 compared to control. The dressing percentage showed significant (p<0.05) difference among treatment groups and control. Organs weight were similar among treatment groups, although the breast meat and bursa weight differed significantly (p<0.05) in treatment groups. A significant increase (p<0.05) in meat crude protein (CP) and total ash content in treatment groups relative to control. Meat thiobarbituric acid reactive substances (TBARS) significantly (p<0.05) decreased at 0.8%, 1.2% and 1.8% during 1st, 2nd, 3rd weeks and in average value as well. Net return and benefit cost ratio (BCR) substantially increased in all treatment groups compared to control (p<0.05). Finally, dietary OLP supplementation improved growth performance, meat CP and ash content, increased blood cholesterol, HDL content, net rutrun and BCR while reduced meat TBARS value. Thus, olive leaf probiotics can be a potential source to be used as feed additive in broiler. Asian J. Med. Biol. Res. December 2020, 6(4): 650-658

Total 288 Ross-308 male broiler chicks were randomly distributed in a complete block design at 2×3 (Two different phytochemicals, i.e., Azadirachta indica, and Curcuma longa at three different levels, i.e., 0, 0.063, and 0.125% of the basal diet) factorial arrangement. Final live weight (FLW), average daily feed intake (ADFI), average daily gain (ADG), feed efficiency (FE), carcass characteristics, cardio-pulmonary morphometry, haemato-biochemical indices, gut morphology, ileal nutrient digestibility, tibia morphometry, meat quality and fatty acid profile were measured. Results indicated that, supplementation of Azadirachta indica leaf meal (AILM) decreased the FLW, ADFI, ADG, gizzard weight, right ventricular diameter and increased the FE, tibia calcium content, left ventricular weight and the ratio of right and left ventricle. The AILM substantially increased the malonaldehyde concentration in the Pectoralis major muscle of the broiler chicken at 7th and day 14th days. The Curcuma longa powder (CLP) decreased the FLW, ADFI and ADG without affecting the FE and increased the weight of right ventricle, left ventricle and tibia length. The AILM and CLP interacted to decrease the ADFI, total saturated fatty acid content and increase the FE, ω-6, ω-9, total unsaturated fatty acids, total poly-unsaturated fatty acids and the ratio of total unsaturated: saturated fatty acid in the breast muscle of the broiler chicken. It was concluded that Azadirachta indica and Curcuma longa in combination with fish oil improved the FE and meat quality of the broiler chicken at the expense of ADFI and ADG.

Total 288 Ross‐308‐day‐old male broiler chicks were randomly distributed into six dietary treatment groups in a two‐way ANOVA with 2 × 3 factorial arrangements (two factors, i.e., dietary protein and energy having two types of protein, e.g., plant, animal and three different sources of energy, e.g., soybean oil, rice bran oil and sunflower oil) to justify if animal protein‐soybean oil based broiler diet optimizes net profit at the expense of desirable ω‐6 fatty acids in the breast muscle of the broiler chicken. Average daily feed intake (ADFI), final live weight (FLW), average daily gain (ADG), feed efficiency (FE), carcass characteristics, cardio‐pulmonary morphometry, fatty acid profile of the breast muscle and cost‐benefit analysis were measured. Results indicated that animal protein significantly increased 4.27% FLW, 6.13% ADFI, 4.31% ADG and 2.93% wing weight. Accordingly, soybean oil increased 4.76% FLW, 3.80% ADG and 1.36% dressing percentage at the expense of 12.07% proventriculus weight compared with sunflower oil. The generalized linear model identified no interaction effects of the sources of protein and energy on overall performance of the birds. Replacement of vegetable protein by animal protein decreased 14.01% ∑ω‐3, 12.16% ∑ω‐6 and 12.21% sum of polyunsaturated fatty acids (∑PUFA) and concomitantly increased 10.82% sum of saturated fatty acids (∑SFAs) in the breast muscle (Pectoralis major). Accordingly, replacement of sunflower oil by soybean oil decreased 29.17% ∑ω‐3, 6.71% ∑ω‐6, 11.62% sum of monounsaturated fatty acids (∑MUFAs) and 7.33% ∑PUFAs and concurrently increased 18.36% ∑SFAs in the breast muscle of the broiler birds. It was concluded that animal protein‐soybean oil‐based broiler diet optimized net profit at the expense of desirable ω‐3 and ω‐6 fatty acids in the breast muscle of the broiler chicken.