Egg production in the copepod Acartia tonsa was evaluated using different densities of the microalgae Thalassiosira weissflogii, Chaetoceros muelleri and Isochrysis galbana. Male and female were kept under controlled conditions (salinity 30, 20 • C, photoperiod 12L:12D), acclimated to the experimental conditions and left over a period of 24 h to allow copulation. Algal densities tested were equivalent in biovolume and corresponded to 0, 2.5, 5, 10, 20, 40 and 60.10 3 cells.mL −1 of T. weissflogii. Ten acclimated female were separated, transferred to glass bottles and exposed for further 24 h to the corresponding experimental medium. After this period, the eggs were fixed and counted. Copepod egg production reached a threshold value when T. weissflogii, C. muelleri and I. galbana were supplied at 10.10 3 , 140.10 3 and 640.10 3 cells.mL −1 , respectively. Mean egg production corresponded to 28.0 ± 0.5, 20.1 ± 1.0 and 22.0 ± 3.5 eggs.female −1 .day −1 , respectively. Copepods fed T. weissflogii showed the highest mean egg production while those fed I. galbana reached a maximum egg production when the algae were supplied at a density two-to fourfold higher, considering the biovolume of T. weissflogii and C. muelleri. These differences are explained considering the different sizes of the microalgae used to feed the copepods.
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