Human ribosomal proteins play important structural and functional roles in the ribosome and in protein synthesis. An efficient method to recombinantly produce and purify these proteins would enable their full characterisation. However, the production of human ribosomal proteins can be challenging. The only published method about the recombinant production of human ribosomal proteins involved the recovery of proteins from inclusion bodies, a process that is tedious and may lead to significant loss of yield. Herein, we explored the use of different Escherichia coli competent cells and fusion protein tags for the recombinant production of human ribosomal proteins. We found that, by using thioredoxin as a fusion protein, soluble ribosomal protein could be obtained directly from cell lysates, thus leading to an improved method to recombinantly produce these proteins.

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Hypoxia‐inducible factor prolyl hydroxylase domain 2 (PHD2) is an important oxygen sensor in animals. By using the CO‐releasing molecule‐2 (CORM‐2) as an in situ CO donor, we demonstrate that CO is an inhibitor of PHD2. This report provides further evidence about the emerging role of CO in oxygen sensing and homeostasis.

Explaining antimicrobial battacin lipopeptides by investigating the solution structure – the propensity to aggregate may have a role in a declined antimicrobial activity.

The development of adaptive nanomaterials that are responsive to changes in their surrounding environment would enable such materials to be used in wide range of applications such as drug delivery vehicles or biosensors. Reversible boronic ester chemistry, which is used in this work, has several advantages as a building block for making adaptive nanomaterials including the ease of preparation, high sensitivity to external stimuli such as pH, and relative stability especially when compared to other non-covalent reversible systems. Herein, by using small boronic acids as anchor and peptides as connectors, we report progress in the initial development of novel, peptidyl-based pH dependent adaptive nanomaterials using reversible boronic ester chemistry and its characterisation using small angle X-ray scattering.