A novel e-waste-degrading strain, PE08T, was isolated from contaminated soil collected from a paper mill yard in Lalkuan, Uttarakhand, India. Strain PE08T was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-positive. Optimum growth was observed at 30 °C (range, 5–40 °C), with 1–2 % NaCl (range, 0–3 %) and at pH 7 (range 6–11). The phylogeny based on 16S rRNA gene sequences delineated strain PE08T to the genus Pseudomonas and showed highest sequence similarity to Pseudomonas furukawaii KF707T (98.70 %), followed by Pseudomonas aeruginosa DSM 50071T (98.62 %) and Pseudomonas resinovorans DSM 21078T (97.93 %). The genome of strain PE08T was sequenced and had one scaffold of 6056953 bp, 99.84 % completeness and 182× coverage were obtained. The G+C content in the genome was 64.24 mol%. The DNA–DNA hybridization and average nucleotide identity values between strain PE08T and its closely related type strain, P. resinovorans DSM 21078T were below 34.8 % and 87.96 %, respectively. The phylogenetic analysis based on whole-genome sequence and concatenated GyrB and RpoB proteins revealed that strain PE08T forms a district clade in the family Pseudomonadaceae . The predominant fatty acids were summed feature 8 (C18 :  1ω7c and/or C18 :1 ω6c), summed feature 3 (C16 :  1ω7c and/or C16 :  1ω6c), C16 : 0 and C12 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The phenotypic, chemotaxonomic and genetic analysis, including overall genome relatedness index values, indicated that strain PE08T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas lalkuanensis sp. nov. is proposed. The type strain is PE08T (=MCC 3792=KCTC 72454=CCUG 73691).

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The Indian Himalayan Region (IHR) is the center of the diverse food culture comprising fermented and non-fermented ethnic foods and alcoholic beverages. Diverse tribal communities in IHR (Uttarakhand, Himachal Pradesh, Jammu and Kashmir, Laddakh, Sikkim, Assam, Arunachal Pradesh, Manipur, Meghalaya, and Tripura) have been long known for their rich culture and food habits. Having strong ritual importance among the ethnic people of the IHR, alcoholic beverages are being consumed in various cultural, social, and religious events for ages. Consumption of in-house prepared alcoholic beverage is the socio-cultural tradition in India as well as across the globe. The processes and ingredients involved in alcoholic beverage preparations vary with raw material availability in different regions. The majority of the fermented drinks are cereal-based with a significant proportion of various plants and fruits as the main raw material, making a beverage more unique in taste. Some plant ingredients used for traditional alcoholic beverages have potential nutraceutical as well as therapeutic properties that are well documented. These properties could constitute an additional economic value for traditional alcoholic beverages commercialization, which, in turn, could promote the local rural economy. Until now, such beverages have only received marginal attention by ethnobotanists and few studies concern traditional fermented beverages in the IHR. In this view, the current review focused on preparation, diversity, cultural, and economic significance and health benefits of ethnic beverages used by tribal communities in the IHR.

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Application of polyhydroxybutyrate (PHB) to plastic industry has expanded over the last decades due to its attracting features over petro-based plastic, and therefore, its waste accumulation in nature is inevitable. In the present study, a total of four bacterial strains, viz., MK3, PN12, PW1, and Lna3, were formulated into a consortium and subsequently used as biological tool for degradation of biopolymers. The consortium was tested through λ shifts under in vitro conditions for utilization of PHB as sole carbon source. Talc-based bioformulations of consortium were used for the degradation of PHB film composites under in situ conditions. After 9 months of incubation, the recovered samples were monitored through Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM), respectively. Analytical data, viz., changes in λ shifts (212-219 nm), FT-IR spectra, and SEM micrographs, revealed the biodegradation potential of developed consortium against PHB film composites, i.e., higher degradation of copolymer films was found over blend films. The used consortium had enhanced the rate of natural degradation and can be further used as a natural tool to maintain and restore global environmental safety.

Thermal stress either cold or heat stress is one of the major factors that influence microorganisms, and to survive from these adverse conditions, microorganisms have to adapt different survival strategies. Some major survival strategies adapted by the microorganisms in response to cold stress are metabolic adaptations, change in cell membrane structure and functions such as membrane fluidity, molecular adaptations that includes change in gene expression, production of cold-adaptive enzymes, and the production of compounds like cryoprotectants that protects microorganisms from these adverse effects. These survival strategies represent bacterial adaptations, and those microorganisms that have potential to adapt better in these survival conditions are most likely to survive in cold stress. Cold-adaptive microorganisms possess high survival instincts and simultaneously offer numerous advantages in pursuits of biotechnological advances. This advancement includes production of cold-adaptive enzymes and proteins, and these proteins and enzymes are very important for commercial purpose that contribute to Indian economy too.Hemant Dasila and Damini Maithani contributed equally with all other contributors.

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