Mentha × piperita L., also known as peppermint, is a plant with various uses, including medicine, cosmetics, and food. Numerous industries have a high need for peppermint products, but Indonesia is currently unable to meet this demand and should continue to import peppermint. One effort can be made to improve cultivation procedures, and tissue culture becomes one alternative. This research uses shoots as explants with Murashige & Skoog's basic media and growth regulators BAP and NAA. The research was conducted in two phases: six-week initial planting and seven-week subculture. The treatment of BAP 4 mg/L + NAA 0.5 mg/L provides better performance for the number of leaves, and BAP 3 mg/L produced the best response regarding the number of shoots. Furthermore, BAP 1 mg/L + NAA 1 mg/L produced the best response to shoot height and number of leaves, and BAP 3 mg/L + NAA 0.5 mg/L generated the best response to root length. Based on the research, BAP 3 mg/L is the optimal treatment.
Nurseries are the main critical phase in the cultivation of Coleus (Plectranthus scutellarioides (L.) R.Br.), which is useful as a raw material for traditional medicine. The key to producing well-grown, healthy, and uniform seedlings is using exogenous hormones throughout the seedling stage. Shallot has the potential to be used as an exogenous hormone. The study was conducted with shallot concentration treatment (0, 35, 70, and 100%) and soaking time (0, 12, and 24 hours). The results show shallot's activity as an exogenous hormone in coleus seedlings, including increasing the root volume of coleus seedlings through the initiation of root hairs, altering seedling height, and stimulating seedling adventitious shoot growth. Conversely, high concentrations of shallots reduced the number of coleus seedling shoots.
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