Molecular diversity analysis of Indian and Exotic genotypes of Brassica juncea genotypes was carried out using simple sequence repeat (SSR) markers. Sixteen out of 32 SSR markers were found to be polymorphic and amplified 54 alleles in 23 genotypes with an average of 2.37 and 0.31 for alleles per locus and polymorphic information content (PIC), respectively. The genotypes were grouped into three distinct clusters based on unweighted neighbour joining and population structure analyses. The molecular analysis revealed that markers obtained from diploid progenitors were useful for molecular diversity studies in Brasicca juncea.
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