aims: To investigate anticancer activity of sericin preparation from cocoons of A. proylei. background: In spite of much progress in understanding the biology of cancer disease, advancement in technology for early diagnosis, the expanding array of anticancer drugs and treatment modalities, the global cancer burden is still significant and increasing. It is estimated that the new cases of cancer in the year 2040 will be 29.4 million per year globally. Sericin, an adhesive protein of silk cocoons has been shown to be a potential protein in various biomedical applications including cancer therapeutics. The present study evaluates the anticancer property of sericin from cocoons of Antheraea proylei J (SAP) against human lung cancer (A549) and cervical cancer (HeLa) cell lines. This is the first report of anti-cancer activity of the non-mulberry silkworm A. proylei J. objective: 1. Establish antiproliferative potential of SAP. 2. Identify the molecular mechanism of cell death induced by SAP on two different cell lines. method: SAP was prepared from cocoons of A. proylei J. by the process of degumming method. The amino acid composition of SAP was determined by HPLC. Cytotoxicity activity was assessed by MTT assay and genotoxicity activity was assessed by comet assay. Cleavage of caspase and PARP proteins and phosphorylation of MAPK pathway members were analysed by Western blotting. Cell cycle analysis was done by flow cytometer. result: SAP causes cytotoxicity to A549 and HeLa cell lines with the IC50 values 3.8 and 3.9 µg/µl respectively. SAP induces apoptosis in a dose dependent manner through caspase-3 and p38, MAPK pathways in A549 and HeLa cells. Moreover, in A549 and HeLa cells SAP induces cell cycle arrest at S phase in a dose dependent manner. conclusion: The difference in the molecular mechanisms of apoptosis induced by SAP in A549 and HeLa cell lines may be due to the difference in the genotypes of the cancer cell lines. However, further investigation is warranted. The overall results of the present study envisage the possibility of using SAP as anti-tumorigenic agent. other: This is the first report of sericin preparation from A. proylei to show anticancer activity. Other reports were from domesticated silkworms.
BackgroundDespite much progress in understanding the biology of cancer disease, advancement in technology for early diagnosis, the expanding array of anticancer drugs, and treatment modalities, the global cancer burden is still significant and increasing. It is estimated that the new cases of cancer in the year 2040 will be 29.4 million per year globally. Sericin, an adhesive protein of silk cocoon, is a potential protein in various biomedical applications including cancer therapeutics. The present study evaluates the anticancer property of sericin prepared from cocoons of Antheraea proylei J. (A. proylei ) against human lung cancer (A549), cervical cancer (HeLa), and prostate cancer (PC3) cell lines. This is the first report of the anti-cancer activity of the non-mulberry silkworm A. proylei. MethodsSericin preparation (SP) was prepared from cocoons of A. proylei J. by the process of the degumming method. The amino acid composition of the SP was determined by HPLC. Cytotoxicity activity was assessed by MTT assay and genotoxicity activity was assessed by comet assay. Cleavage of caspase and PARP proteins and phosphorylation of MAPK pathway members were analyzed by Western blotting. Cell cycle analysis was done by FACS flow cytometry.Results SP causes cytotoxicity to A549, HeLa, and PC3 cell lines with the IC50 values ranging from 3.4-3.9 µg/µl. SP induces apoptosis in a dose-dependent manner through caspase-3 and p38/SAPK/ERK pathways in A549 and HeLa cells whereas in PC3 cells SP induces apoptosis independent of caspase but through p38 pathway. Moreover, in the case of A549 and HeLa cells, SP induces cell cycle arrest at the S phase whereas at the G0 phase in the case of PC3 cells in a dose-dependent manner.ConclusionThe difference in the molecular mechanisms of apoptosis induced by SP in A549 and HeLa cell lines, and in PC3 cell lines may be due to the difference in the genotypes of the cancer cell lines where A549 and HeLa cells are being non-malignant and p53 positive whereas PC3 cell is being malignant and p53 negative. The overall results of the present study envisage the possibility of using SP as an anti-tumorogenic agent.
Sericin is a byproduct of silk fibre industries with potential applications in therapeutics and cosmetic industries. Sericin protein differs in amino acid composition pertaining to the habitat and species of the silkworm. Though antiproliferative activity of sericin from B. mori is documented, sericin from A. proylei remains largely unexplored on various cell lines. The study envisages use of sericin protein as an anticancer agent in prostate cancer by using PC3 cells as a model. The study reports a caspase-3 and PARP independent cell death mechanism in PC3 cells treated with sericin and its ability to induce apoptosis through activation of p38 and arrests of cell cycle at G0/G1 phase.
BackgroundIn spite of much progress in understanding the biology of cancer disease, advancement in technology for early diagnosis, the expanding array of anticancer drugs and treatment modalities, the global cancer burden is still significant and increasing. It is estimated that the new cases of cancer in the year 2040 will be 29.4 million per year globally. Sericin, an adhesive protein of silk cocoons has been shown to be a potential protein in various biomedical applications including cancer therapeutics. The present study evaluates the anticancer property of sericin from cocoons of Antheraea proylei J. (SAP) against human lung cancer (A549), cervical cancer (HeLa), and prostate cancer (PC3) cell lines. This is the first report of anti-cancer activity of the non-mulberry silkworm A. proylei J. Methods SAP was prepared from cocoons of A. proylei J. by the process of degumming method. The amino acid composition of SAP was determined by HPLC. Cytotoxicity activity was assessed by MTT assay and genotoxicity activity was assessed by comet assay. Cleavage of caspase and PARP proteins and phosphorylation of MAPK pathway members were analysed by Western blotting. Cell cycle analysis was done by flow cytometery.ResultsSAP causes cytotoxicity to A549, HeLa and PC3 cell lines with the IC50 values ranging from 3.4-3.9 µg/µl. SAP induces apoptosis in a dose-dependent manner through caspase-3 and p38 and ERK pathways in A549 and HeLa cells respectively whereas in PC3 cells, SAP induces apoptosis independent of caspase through p38 pathway. Moreover, in the case of A549 and HeLa cells SAP induces cell cycle arrest at S phase in a dose dependent manner whereas at G0 phase in the case PC3 cells.ConclusionSAP induces apoptosis in A549, HeLa, and PC3. The difference in the molecular mechanisms of apoptosis induced by SAP in A549 and HeLa and in PC3 may be due to the differences in the genotypes of the cancer cell lines. However, further investigation is warranted. The overall results of the present study envisage the possibility of using SAP as anti-tumorigenic agent.
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