In the recent decades, nanotechnology is gaining tremendous impetus due to its capability of modulating metals into their nanosize, which drastically changes the chemical, physical, biological and optical properties of metals. In this study, silver nanoparticles (AgNPs) synthesis using aqueous leaf extracts of Tagetes patula L. which act as reducing agent as well as capping agent is reported. Synthesis of AgNPs was observed at different parameters like temperature, concentration of silver nitrate, leaf extract concentration and time of reduction. The AgNPs were characterized using UV-vis spectroscopy, scanning electron microscope with energy dispersive spectroscopy, transmission electron microscopy with selected area electron diffraction, X-ray diffraction, Fourier transform infrared and dynamic light scattering analysis. These analyses revealed the size of nanoparticles ranging from 15 to 30 nm as well revealed their spherical shape and cubic and hexagonal lattice structure. The lower zeta potential (-14.2mV) and the FTIR spectra indicate that the synthesized AgNPs are remarkably stable for a long period due to the capped biomolecules on the surface of nanoparticles. Furthermore, these AgNPs were found to be highly toxic against phytopathogenic fungi Colletotrichum chlorophyti by both in vitro and in vivo and might be a safer alternative to chemical fungicides.
SSR hamper successful cultivation and causes significant yield losses globally including India. The SSR disease incidence ranged from 3.59 to 18.50 per cent in mustard growing areas of Rajasthan and Bhartpur district having the highest (18.50%) disease incidence. However, the Udaipur district had the lowest (3.59%) disease outbreak. SSR was responsible to cause 18.80 per cent overall yield losses in two consecutive years (rabi 2016-17 and 2017-18). Highest yield loss (43.82%) was observed from Bhartpur district while minimum yield loss (3.51%) had recorded from Udaipur district. Characteristics symptoms, formation of white mycelium on host stem and development of sclerotia on/inside the stem, of SSR on mustard crop were noted during survey.
Downy mildew (DM) caused by Peronospora arborescens is the most alarming disease of opium poppy which hampered the production of opium crop in major growing areas of India. The pooled data taken from Rabi 2016-17 and 2017-18 demonstrated that chemical protected un-inoculated plot had a minimum per cent disease severity (9.83) with maximum dry latex yield (31.25 kg ha-1), seed yield (801.31 kg ha-1) and husk yield (889.66 kg ha-1). However, plots inoculated with Peronospora arborescens at high inoculum density of 9×105 spores ml-1 had considerably higher per cent disease severity (67.00) and minimum dry latex yield (6.94 kg ha-1), seed yield (548.42 kg ha-1) and husk yield (590.86 kg ha-1) with maximum 77.79,31.56 and 33.58 per cent loss as compared to un-inoculated chemical protected plot, respectively. The severity of the downy mildew disease was found to rise in direct conflict with the level of inoculum concentration with significant reduction in dry latex yield, seed yield and husk yield.
SSR hamper successful cultivation and causes significant yield losses globally including India. The SSR disease incidence ranged from 3.59 to 18.50 per cent in mustard growing areas of Rajasthan and Bharatpur district having the highest (18.50%) disease incidence. However, the Udaipur district had the lowest (3.59%) disease outbreak. SSR was responsible to cause 18.80 per cent overall yield losses in two consecutive years (Rabi 2016-17 and 2017-18). Highest yield loss (43.82%) was observed from Bharatpur district while minimum yield loss (3.51%) had recorded from Udaipur district. Characteristics symptoms, formation of white mycelium on host stem and development of sclerotia on/inside the stem, of SSR on mustard crop were noted during survey.
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