As antibiotic resistance is becoming a major problem nowadays in a treatment of infections, bacteriophages (also known as phages) seem to be an alternative. However, to be used in a therapy, their life cycle should be strictly lytic. With the growing popularity of Next Generation Sequencing (NGS) technology, it is possible to gain such information from the genome sequence. A number of tools are available which help to define phage life cycle. However, there is still no unanimous way to deal with this problem, especially in the absence of well-defined open reading frames. To overcome this limitation, a new tool is definitely needed. We developed a novel tool, called PhageAI, that allows to access more than 10 000 publicly available bacteriophages and differentiate between their major types of life cycles: lytic and lysogenic. The tool included life cycle classifier which achieved 98.90% accuracy on a validation set and 97.18% average accuracy on a test set. We adopted nucleotide sequences embedding based on the Word2Vec with Ship-gram model and linear Support Vector Machine with 10-fold cross-validation for supervised classification. PhageAI is free of charge and it is available at https://phage.ai/. PhageAI is a REST web service and available as Python package. Machine learning and Natural Language Processing allows to extract information from bacteriophages nucleotide sequences for lifecycle prediction tasks. The PhageAI tool classifies phages into either virulent or temperate with a higher accuracy than any existing methods and shares interactive 3D visualization to help interpreting model classification results.

Camera traps are used worldwide to monitor wildlife. Despite the increasing availability of Deep Learning (DL) models, the effective usage of this technology to support wildlife monitoring is limited. This is mainly due to the complexity of DL technology and high computing requirements. This paper presents the implementation of the light-weight and state-of-the-art YOLOv5 architecture for automated labeling of camera trap images of mammals in the Białowieża Forest (BF), Poland. The camera trapping data were organized and harmonized using TRAPPER software, an open-source application for managing large-scale wildlife monitoring projects. The proposed image recognition pipeline achieved an average accuracy of 85% F1-score in the identification of the 12 most commonly occurring medium-size and large mammal species in BF, using a limited set of training and testing data (a total of 2659 images with animals).Based on the preliminary results, we have concluded that the YOLOv5 object detection and classification model is a fine and promising DL solution after the adoption of the transfer learning technique. It can be efficiently plugged in via an API into existing web-based camera trapping data processing platforms such as e.g. TRAPPER system. Since TRAPPER is already used to manage and classify (manually) camera trapping datasets by many research groups in Europe, the implementation of AI-based automated species classification will significantly speed up the data processing workflow and thus better support data-driven wildlife monitoring and conservation. Moreover, YOLOv5 has been proven to perform well on edge devices, which may open a new chapter in animal population monitoring in real-time directly from camera trap devices.

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