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The aim of this study was to determine the percentage of participation of particular species of microorganisms, isolated from the uterus of cows with endometritis and from cows without inflammatory lesions of the uterus, in the same postpartum period. The aim of the study was also to examine how long after parturition non-treated endometritis persists. Moreover, antibiotic susceptibility tests were carried out of the bacterial isolates dominating in the uterus.

The aim of the study was to evaluate the concentrations of proinflammatory cytokines: tumor necrosis factor (TNF-α) and interleukin-6 (IL-6), anti-inflammatory cytokine interleukin-10 (IL-10), and acute phase proteins (APPs)—haptoglobin (Hp) and serum amyloid A (SAA) in serum and uterine washings of cows with subclinical endometritis, and compare them to healthy animals. The study was performed on 24 cows on day 60 after delivery. The cows were divided into two groups based on the results of cytological tests: 12 cows with subclinical endometritis and 12 healthy cows. Experimental material consisted of blood serum and uterine washings. The levels of the following cytokines in the study material were determined with ELISA: TNF-α, IL-6, IL-10 and APPs - Hp and SAA. The results show that the levels of TNF-α (p < 0.01), IL-6, IL-10 as well as SAA and Hp were significantly higher in the serum of cows with subclinical endometritis compared to the controls (p < 0.001). Uterine washings had significantly higher levels of IL-6, IL-10, and Hp in the experimental cows compared to the controls (p < 0.001). The demonstrated differences in the concentration of cytokines and APP between cows with subclinical endometritis and healthy cows, in both the serum and uterine washings, may suggest the usefulness of these parameters in the diagnosis of subclinical endometritis in cows in the late postpartum period.

This paper presents the study on determination of the free amino acid in blood serum of cows with high milk production (Herd A) and cows with subclinical ketosis compared to healthy ones (Herd B).  In Herd A examinated 12 cows in the first 100  days in milk. A total of 24 cows  from a herd B divided into two groups: experimental (12 cows with ketosis) and control (12 healthy cows) were included in the study. Statistically significantly higher concentrations of glutamine, glutamic acid, isoleucine (p ≤ 0.001), and tyrosine (p ≤ 0.05) were found in dairy cows with subclinical ketosis compared to healthy ones. A significant decrease in the concentrations of asparagine, histidine, methionine, and serine (p ≤ 0.001) as well as alanine, leucine, lysine and proline (p ≤ 0.05) was observed. In Herd A was high level of total essential amino acids  in blood serum. In our study, the changes, in particular, observed in amino acid concentration in cows with subclinical  ketosis indicate its intensive use in both ketogenesis and gluconeogenesis processes.