PAGE 4293:Fig . 3A (upper right panel) shows an alteration of a lane (10 M PD98059) that was not detected at the time of manuscript submission and that is contrary to the Journal of Biological Chemistry guidelines. Herein, we provide an alternative figure that shows that the ERK inhibitor PD98059 inhibited Vpr-(52-96)-induced ERK activation in a dose-dependent manner. THP-1 cells were treated with the indicated concentrations of PD98059 for 4 h, followed by stimulation with 1.5 M Vpr-(52-96) peptide for another 2 h. The legend of this figure remains unchanged. The data support the published observations and therefore do not impact the interpretation of this figure or the central conclusion of this article. During the continuation of our work on hsc70 trafficking, we have noticed that there was an error during the preparation of midiprep plasmid DNA for two constructs that were described in this work. The error reported by us does not alter the validity of the raw data. The following two constructs were affected by the error: GFP-hsc70(225-262) and GFP-hsc70(245-287). These two plasmid DNAs have been inverted in our article. Following the detection of the error, we have verified the correctness of all other constructs encoding wild-type or mutant fragments of domain IIB of hsc70. This error affects the presentation of results shown in Figs. 2B and 3 (A and C) and the interpretation depicted in Fig. 6A. Thus, the correct interpretation of our data is that segment 245-287 locates constitutively in the nucleolus under nonstress and stress conditions. Fragment 225-262 displays weak stressinduced nucleolar accumulation. This makes residues 225-244 the negative regulator of hsc70 nucleolar accumulation. The experiments reported were carried out at 390 mM methyl--cyclodextrin (MCD; 825 mg ϩ 1 ml of water, which gives a volume of ϳ1.6 ml), not as reported at 625 mM MCD (825 mg/ml of solution). We (MiJin Son and E. L.) have investigated asymmetric vesicle preparation at the higher MCD concentration and found that 625 mM MCD could be used to produce asymmetric small unilamellar vesicles. However, the results were not as reproducible as with the lower MCD concentration. We recommend the use of 390 mM MCD to prepare asymmetric small unilamellar vesicles. The first sentence in the Abstract should read as follows: The cyclic dinucleotide c-di-AMP synthesized by the diadenylate cyclase domain was discovered recently as a messenger molecule for signaling DNA breaks in Bacillus subtilis.In the Introduction, line 21 in the right-hand column should read as follows: This group of proteins (COG3887), as represented by the B. subtilis protein YybT, contains two N-terminal transmembrane helices, a region that shares minimum sequence homology with some PerArnt-Sim (PAS) domains, a highly modified GGDEF domain, and a DHH/DHHA1 domain (see Fig. 1). ADDITIONS AND CORRECTIONS This paper is available online at www.jbc.orgWe suggest that subscribers photocopy these corrections and insert the photocopies in the original publication at ...
The present submission focuses on the concept of stand-off annotation as it is implemented in the current version of the TEI Guidelines. We look at the motivation for choosing the stand-off approach to encoding Language Resources, briefly recount the history of the concept within the broadly conceived TEI setting (since TEI P3 and the LT NSL suite, through CES and XCES, ending in TEI P5), review the various kinds of hyperlink semantics and identify three kinds of reasons for the poor uptake of the TEI-recommended stand-off annotation approach to corpus encoding. We also suggest some solutions that may contribute to a change in the current state of affairs.
We report on a project which we believe to have the potential to become home to, among others, bilingual dictionaries for African languages. Kept in a well-structured XML format with several possible degrees of conformance, the dictionaries will be able to get usable even in their early versions, which will be then subject to supervised improvement as user feedback accumulates. The project is FreeDict, part of SourceForge, a well-known Internet repository of open source content. We demonstrate a possible process of dictionary development on the example of one of FreeDict dictionaries, a Swahili-English dictionary that we maintain and have been developing through subsequent stages of increasing complexity and machineprocessability. The aim of the paper is to show that even a small bilingual lexical resource can be submitted to this project and gradually developed into a machineprocessable form that can then interact with other FreeDict resources. We also present the immediate benefits of locating bilingual African dictionaries in this project. We have found FreeDict to be a very promising project with a lot of potential, and the present paper is meant to spread the news about it, in the hope to create an active community of linguists and lexicographers of various backgrounds, where common research subprojects can be fruitfully carried out.
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