A simple, precise, and accurate isocratic RP-UFLC stability-indicating assay method has been developed to determine trospium chloride in tablet dosage form. Isocratic separation was achieved on an Enable-C18G (250 mm × 4.6 mm i.d., particle size 5 μm) column at room temperature, the mobile phase consisted of acetonitrile:0.01M TBAHS (50:50, v/v) at a flow rate of 1.0 ml/min, the injection volume was 20 μl, and PDA detection was carried out at 215 nm. The drug was subjected to acid and alkali hydrolysis, oxidation, photolysis, and heat as stress conditions. The method was validated for specificity, linearity, precision, accuracy, robustness, and system suitability. The method was linear in the drug concentration range of 10–300 μg/ml with the correlation coefficient being 0.999. The RSD for repeatability and intermediate precision was well below 2%. The mean recoveries were between 100.52–101.68% for trospium chloride.
Allergic rhinitis (AR) is a heterogeneous disorder that despite its high prevalence is often undiagnosed. It is characterized by one or more symptoms including sneezing, itching, nasal congestion and rhinorrhea. Many causative agents have been linked to allergic rhinitis (AR) including pollens, molds, dust mites and animal dander allergy. Montelukast and bilastine are used in the treatment of allergic rhinitis (AR). Montelukast and bilastine each drug reveals in vivo and in vitro. They are generally administrated as tablets. Determination of montelukast and bilastine in bulk dosage form, tablet dosage form and pharmaceutical dosage form. Method indicating human plasma stability and impurity profiling are also described for Montelukast and Bilastine drugs. Several analytical methods including UV, HPTLC and HPLC method has been developed. Estimation of montelukast and bilastine for quantitative and qualitative method can be used. In this review methods for determination of montelukast and bilastine in alone and in combination by UV and RP-HPLC techniques. This review covers most recent analytical methods such as various spectroscopic methods, chromatographic methods and other methods for determination of Montelukast and Bilastine in various pharmaceutical dosage forms. The following study illustrate the review on analytical method which includes estimating the anti-histamines drugs.
A novel, accurate and precise RP-UFLC method for determination of xanthinol nicotinate has been developed and validated. Separation was achieved on an Enable C 18 G column (250 mm × 4.6 mm i.d., 5 μm) using acetonitrile: 10 mM TBAHS (40:60, v/v) as mobile phase at a flow rate of 0.8 mL/min and PDA detection at 274 nm. Linearity was observed in the concentration range of 1.0-60 μg/mL (r 2 =0.999). The method was validated for accuracy, precision, stability, specificity, robustness and system suitability. Forced degradation was performed by using HCl, NaOH, H 2 O 2 , thermal and UV radiation. The method was used successfully for the determination of xanthinol nicotinate in tablet dosage form.
A simple, selective and rapid reversed phase High Performance Liquid Chromatographic (RP-HPLC) method has been developed and validated for the simultaneous analysis Gatifloxacin and flurbiprofen sodium in eye drops. The separation was carried out using a mobile phase consisting ACN: Buffer (pH 3.5) in the ratio of 55:45 v/v. The column used was Phenomenex luna ODS C18 (250mm X 4.6 mm i.d., 5 μm particle size) with flow rate of 1 ml / min using UV detection at 268 nm. The described method was linear over a concentration range of 2-12 μg/ml for both of Gatifloxacin and flurbiprofen sodium. The retention times of Gatifloxacin and flurbiprofen sodium were found to be 3.710 min. and 6.797 min respectively. Method was validated statistically and recovery studies were carried out. The proposed method has been applied successfully to the analysis of cited drugs either in pure form or in pharmaceutical formulations with good accuracy and precision. The method here in described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.
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