Phytophthora sojae is a devastating pathogen of soybean [Glycine max (L.) Merr.] that causes Phytophthora root and stem rot (PRR) on soybean plants worldwide. The pathogen leads to evident differentiation of pathogenicity which brings some difficulties to the control of the disease. Soybean germplasm plays an important role in disease resistance to P. sojae. To understand the role of soybean in the pathogen differentiation of P. sojae, transcriptome sequencing and biological information technology were used to analyse the difference in transcriptional level of soybean response to P. sojae infection by two isolates BB8 and BZ9 with different pathogenicity. A total of 1596 differentially expressed genes (DEGs) were screened from the soybean plants treated with the strong pathogenic isolate BB8 and weak pathogenic isolate BZ9, of which 256 were upregulated and 1340 were downregulated in BZ9 treated samples. Gene Ontology analysis revealed that the highly enriched terms annotated in biological processes were metabolic process, cellular process and single-organism process. Kyoto Encyclopedia of Genes and Genomes pathways were distributed primarily in metabolism, environmental information processing and cellular processes. Photosynthesisantenna proteins, photosynthesis, phenylpropanoid biosynthesis, carbon fixation in photosynthetic organisms, plant hormone signal transduction, flavonoid biosynthesis, carbon metabolism and nitrogen metabolism were the most substantial metabolic pathways. Ten candidate DEGs were selected and their expression levels were detected via fluorescence quantitative polymerase chain reaction. The expression trends were basically consistent with the results of RNA-seq detection, confirming the reliability of RNA-seq data. Taken together, this was the report on the transcriptome analysis of soybean in response to P. sojae using high-throughput sequencing. A number of DEGs and pathways were discovered, which provided valuable information for obtaining high-quality soybean resistance resources.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.