Ultrafine bubbles
exist in all liquids and are naturally stable.
As their properties are not entirely known, it is unclear how they
impact the surrounding solution and comparable-sized particles within
it. It is essential to further investigate the properties of ultrafine
bubbles in order to expand their industrial application. In this regard,
the effect of ultrafine bubbles on bacterial development is of particular
interest. Our current study, using optical density measurements and
fluorescence microscopic images has demonstrated that ultrafine gas
bubbles impact the morphology and phenotype of Escherichia
coli and Pseudomonas aeruginosa. Specifically, Fourier transform infrared spectroscopic measurements
indicated a thickening of bacterial membranes in samples exposed to
ultrafine bubbles. The study also confirmed that ultrafine bubbles
can inhibit bacterial cell growth. This study signifies the role of
surface phenomena in bacterial culture, which is crucial in the upstream
processes of recombinant DNA technology applications.
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