~ Saccharomyces cererisiue was unable to grow in media containing above about 1.5 mM free sulphite at pH 4.0, whereas Saccharomycodes ludwigiigrew at the same pH value in the presence of 7-8 mM free sulphite. Expressed in terms of pl of intracellular water, the initial velocity of sulphite accumulation by S'codes ludwigii was approximately twice that of S. cererisiae, although the former yeast accumulated at equilibrium only about one-third of the amount of sulphite accumulated by S. cererisiae. A Woolf-Hofstee plot for accumulation of SO-, by S'codes IudNigii at pH 3.0 and 30 "C gave a vertical line. Incorporation of sulphite in growth media induced excretion of acetaldehyde by both yeasts, the rate being greater by S'codes ludwigii than S. cerecisiae. Acetaldehyde excretion was accompanied by release of lower concentrations of pyruvate. Excretion of 2-oxoglutarate was barely detectable. It is suggested that the greater resistance of S'codes ludwigii to sulphite, compared with S. cerecisiae, may be explained partly by its decreased capacity to accumulate the compound, and partly by its ability to produce more acetaldehyde.
Studies on the tricarboxylic acid cycle of Rhodornicrobium vannielii Rm5 demonstrated that, unlike other Rhodospirillaceae, this organism has a functionally incomplete cycle, broken at 2oxoglutarate dehydrogenase, under photoheterotrophic conditions. This enzyme was, however, synthesized when Rm. vannielii was grown under microaerophilic chemoheterotrophic conditions. The citrate synthase exhibited responses to inhibitors characteristic of Gramnegative organisms but, unlike many microbes exhibiting an incomplete cycle, was not inhibited by 2-oxoglutarate. Both NAD-and NADP-linked isocitrate dehydrogenase activity was detectable but the functional roles of these enzymes are unclear. No significant differences in enzyme activities or inhibitor sensitivities of enzymes were detected between heterogeneous cultures and synchronous swarmer cell populations. Ahbreriatiun: TCA, tricarboxylic acid. 0001-2902 0 1986 SGM
We recently studied the fine structure of unusual microorganisms in a urine specimen. To our knowledge, previous electron microscopic investigation of these microorganisms has never been done. The microorganisms were found in the sediments of two separate urine specimens obtained from a 55 year old patient. In these samples, there were yeast cells and two types of gram negative microorganisms; a typical rodlike, non-mobile bacterium, and a spheroid form with two projections. The latter organisms were varying sizes and moved by a typical serpent-like motility. The rod-like structures were approximately 0.4 - 1 μ in diameter and up to 8 μ in length. The spheroid structures, which stained homogeneously and were not spore-like in appearance, were approximately 6 μ in diameter. A few intermediate or transitional forms of various sizes and shapes were also found.
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