Cytochalasin B, a microfilament-altering drug, inhibits lactose synthesis in lactating guinea pig mammary gland [Biochim. Biophys. Acta 392:20, 1975] but not primary by inhibiting glucose transport [Eur. J. Cell Biol. 20:150, 1979]. In order to study the possible role of microfilaments in lactose synthesis and secretion, we isolated both the alveolar (milk-secreting) and myoepithelial (contractile) cells from lactating mammary gland. Light microscopy shows that the alveolar cell fraction (viability approximately 71%) is homogenous and that the cells retain strong polarity of secretory structures in the apical region. Two proteins were extracted from the alveolar cell fraction. One (mol wt 42,000) comigrates with skeletal muscle actin on SDS-PAGE gels. The other, a high-molecular-weight (180,000) protein (HMWP) may be analogous to actin-binding protein or clathrin. An extract from the myoepithelial cell fraction also contains a protein that comigrates with actin but no HMWP. Whole tissue extract contains the 42K protein, and a 185K HMWP. Examination of the alveolar cell extract by electron microscopic (EM) negative staining revealed meshworks of multistranded, interconnecting filaments, with attached globular structures (100-200 A) (possibly the HMWP) and single filaments (40-60 A diameter) branching off. To localize these filamentous structures in situ, whole tissue was glycerinated and incubated with rabbit skeletal muscle heavy meromyosin (HMM). Masses of filaments in myoepithelial cells served as convenient standards for HMM decoration. Decorated filaments have cross-arms or projections, unlike the narrow, smooth filaments of control tissue. Decorated filaments in alveolar cells are located beneath the plasma membrane, in close association with secretory vacuoles, and near the Golgi apparatus; filaments near the latter two are often oriented perpendicular to the plasma membrane. Microvesicles are embedded in meshworks under the plasmalemma and near the Golgi apparatus. Intermediate-sized (85-115 A diameter), non-decorated filaments diverge from the meshworks of decorated filaments. Micro-vesicles are associated with intermediate-sized filaments as well. The association of actin-like filaments with secretory vacuoles and microvesicles and their location in areas of the cell concerned with biosynthetic activities suggest a possible function in the intracellular transport of secretory products.
We have developed a novel approach for quantifying the microstructure of granular thin films using digital image processing and analysis. In the past, conventional scanning electron microscopy of thin films has generated qualitative information on the surface topography and film microstructure. However, when coupled to digital image analysis, the amount or degree of surface contours (i.e., granularity) in SEM micrographs can be quantified in a rapid and reproducible manner. Briefly, SEM micrographs are digitized and the edge boundaries on the film surface are enhanced by a gradient filter; granularity is then quantified by calculating the %AREA covered by the edges with respect to the entire field. Objects of a particular shape, such as phase impurity particles, can be selectively deleted from the image using a specific sequence of shape analysis algorithms and parameter values. In this manner, the contributions of edges from the phase impurity particles is minimized in the final measurement of real surface contours. Statistical analysis of the data yields quantitative information concerning variations in microdomains within single thin films and can detect statistically significant differences among samples. This method is being used in the characterization of the microstructure of superconducting thin films for optimization of their electrical and magnetic properties.
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