The increasing demand
for tissue replacement has encouraged scientists
worldwide to focus on developing new biofabrication technologies.
Multimaterials/cells printed with stringent resolutions are necessary
to address the high complexity of tissues. Advanced inkjet 3D printing
can use multimaterials and attain high resolution and complexity of
printed structures. However, a decisive yet limiting aspect of translational
3D bioprinting is selecting the befitting material to be used as bioink;
there is a complete lack of cytoactive bioinks with adequate rheological,
mechanical, and reactive properties. This work strives to achieve
the right balance between resolution and cell support through methacrylamide
functionalization of a psychrophilic gelatin and new fluorosurfactants
used to engineer a photo-cross-linkable and immunoevasive bioink.
The syntonized parameters following optimal formulation conditions
allow proficient printability in a PolyJet 3D printer comparable in
resolution to a commercial synthetic ink (∼150 μm). The
bioink formulation achieved the desired viability (∼80%) and
proliferation of co-printed cells while demonstrating in vivo immune tolerance of printed structures. The practical usage of existing
high-resolution 3D printing systems using a novel bioink is shown
here, allowing 3D bioprinted structures with potentially unprecedented
complexity.
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