Human sexual behavior research is a multidisciplinary endeavor that seeks to comprehend one of the most vital and complex aspects of our behavioral science. This study aimed to investigate Vietnamese university students' perception of sexual knowledge as well as the relationship between gender, academic year, and sexual orientation. The questionnaire was sent to 666 Vietnamese undergraduate students in four universities which 418 respondents were considered valid for research purposes. The study results showed that there was a significant difference among the four study groups of different (1) academic year; (2) sexual orientations; (3) gender and academic year level; (4) academic year and sexual orientation; (5) gender, academic year, and sexual orientation level when considered jointly on the variables of the three aspects of the Sex knowledge and Attitude Questionnaire II. However, there was no reciprocal relationship between (6) gender; (7) gender, and sexual orientation level. The findings of this study will have implications for school policies that attempt to promote and maintain a positive school climate in an effort to address the issue of sexuality education, including sexual knowledge.
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