The concentration of serum immunoglobulin in different groups of rainbow trout Salrno gairdneri was measured by means of a single radial immuno&ffusion test. The normal IgM concentration was 3.3 mg ml-' in a group of free-living trout. Hypergamrnaglobulinaemia was found in PKDinfected fish and in fish from a trout farm with VHS and ERM, while the IgM concentration was low in sera from fish living under aquarium con&tions. Inhvidual variations were very pronounced. The purity of reference preparations and the specificity of antisera used were examined by crossed in~munoelectrophoresis.
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