Hexavalent chromium is a carcinogenic heavy metal that needs to be removed effectively from polluted aquifers in order to protect public health and the environment. This work aims to evaluate the reduction of Cr(VI) to Cr(III) in a contaminated aquifer through the stimulation of indigenous microbial communities with the addition of reductive agents. Soil-column experiments were conducted in the absence of oxygen and at hexavalent chromium (Cr(VI)) groundwater concentrations in the 1000–2000 μg/L range. Two carbon sources (molasses and EVO) and one iron electron donor (FeSO4·7H2O) were used as ways to stimulate the metabolism and proliferation of Cr(VI) reducing bacteria in-situ. The obtained results indicate that microbial anaerobic respiration and electron transfer can be fundamental to alleviate polluted groundwater from hazardous Cr(VI). The addition of organic electron donors increased significantly Cr(VI) reduction rates in comparison to natural soil attenuation rates. Furthermore, a combination of organic carbon and iron electron donors led to a longer life span of the remediation process and thus increased total Cr(VI) removal. This is the first study to investigate biotic and abiotic Cr(VI) removal by conducting experiments with natural soil and by applying biostimulation to modify the natural existing microbial communities.
Increased groundwater and soil contamination by hexavalent chromium have led to the employment of a variety of detoxification methods. Biological remediation of Cr(VI) polluted aquifers is an eco-friendly method that can be performed in situ by stimulating the indigenous microbial population with organic and inorganic electron donors. In order to study the effect of different redox conditions on microbial remediated Cr(VI) reduction to Cr(III), microcosm experiments were conducted under anaerobic, anoxic, and sulfate-reducing conditions and at hexavalent chromium groundwater concentrations in the 0–3000 μg/L range, with groundwater and soil collected from an industrial area (Inofyta region). As electron donors, molasses, emulsified vegetable oil (EVO), and FeSO4 were employed. To quantitatively describe the degradation kinetics of Cr(VI), pseudo-first-order kinetics were adopted. The results indicate that an anaerobic system dosed with simple or complex external organic carbon sources can lead to practically complete Cr(VI) reduction to Cr(III), while the addition of Fe2+ can further increase Cr(VI) removal rate significantly. Furthermore, Cr(VI) microbial reduction is possible in the presence of NO3− at rates comparable to anaerobic Cr(VI) microbial reduction, while high sulfate concentrations have a negative effect on Cr(VI) bioreduction rates in comparison to lower sulfate concentrations.
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