To assess the long-term anthropogenic load of the Bílina River (Czech Republic), the concentrations of heavy metals and specific organic compounds in different river ecosystem matrices (water, biofilms, and sediments) were determined. Although the current concentrations of pollutants in surface water are low, frequently below the limits of the quantitative analytical methods used, the river ecosystem is still heavily loaded by anthropogenic pollution, mainly from the chemical and mining industries. This was demonstrated by analyzing biofilms and sediments. These matrices are more accurate representatives of the actual situation in the river and do not depend on hydrological conditions or random variability in water quality. The results indicate that the middle and the lower parts of the river are heavily polluted by mercury, arsenic, vanadium, polychlorinated biphenyls, hexachlorobenzene, and dichloro-diphenyl-trichloroethane. As a tributary of the Elbe River, the Bílina River represents a significant risk for the development of quality in this major European river.

The impacts of changes in water temperature and flow on selected water quality parameters, as one of the consequences of climate change, were studied in river catchments in the Czech Republic with little anthropogenic influence. The impact of climate change was manifested by an increase in stream temperature by 1.15°C over 28 years. The selected water quality parameters were dependent on flow, with up to 10-fold increases in the concentrations of ammonia, phosphorus and chlorophyll-a at minimum flow levels. In river catchments with point source pollution predominating, significant pollution with ammonia nitrogen was observed. The influence of increased water temperature compared to flow rates was generally less marked and, with the exception of chlorophyll-a, rather positive. For existing land management and utilization of these river catchments, extreme changes in flow rates will influence the water quality more substantially than the water temperature itself.