The net effect of increased wort osmolarity on fermentation time, bottom yeast vitality and sedimentation, beer flavor compounds, and haze was determined in fermentations with 12 degrees all-malt wort supplemented with sorbitol to reach osmolarity equal to 16 degrees and 20 degrees. Three pitchings were performed in 12 degrees/12 degrees/12 degrees, 16 degrees/16 degrees/12 degrees, and 20 degrees/20 degrees/12 degrees worts. Fermentations in 16 degrees and 20 degrees worts decreased yeast vitality measured as acidification power (AP) by a maximum of 10%, lowered yeast proliferation, and increased fermentation time. Repitching aggravated these effects. The 3rd "back to normal" pitching into 12 degrees wort restored the yeast AP and reproductive abilities while the extended fermentation time remained. Yeast sedimentation in 16 degrees and 20 degrees worts was delayed but increased about two times at fermentation end relative to that in 12 degrees wort. Third "back-to-normal" pitching abolished the delay in sedimentation and reduced its extent, which became nearly equal in all variants. Beer brewed at increased osmolarity was characterized by increased levels of diacetyl and pentanedione and lower levels of dimethylsulfide and acetaldehyde. Esters and higher alcohols displayed small variations irrespective of wort osmolarity or repitching. Increased wort osmolarity had no appreciable effect on the haze of green beer and accelerated beer clarification during maturation. In all variants, chill haze increased with repitching.
Brewery bottom yeast strain 95 from the Pilsner Urquell propagation unit was used to reappraise the efficiency of the acidification power (AP) test consisting in determining the spontaneous (oxygen-induced) and glucose-induced medium acidification caused by yeast and lactic acid bacteria under standard conditions, and used widely for assessing and predicting the vitality of industrial strains. AP was evaluated in yeast stored for different periods of time (0-28 d) at 4 degrees C, at different temperatures before and during the test (0-55 degrees C), and at different concentrations of cells and glucose and different cells-to-glucose ratios. All these factors had a strong effect on acidification kinetics and the AP value. By contrast, the duration of the lag period between yeast collection and the test (0-6 h) had no perceptible effect on the AP value. The best results were achieved at saturation concentrations of cells (> 10 g pressed yeast or approximately 14 g yeast slurry per 100 mL) and glucose (approximately 3 %) and at 25 degrees C. Since an exact evaluation of acidification characteristics depends strongly on the kinetics of the process, the AP test should include monitoring the time course of the acidification.
The optimised acidification power test (APT) of brewer's yeast quality includes storing the yeast slurry at 2°C under beer (AP remains constant for up to 6 days), a 15 min sample equilibration to room temperature, decantation, and washing by triple centrifugation in deionised water. The final yeast pellet keeps its AP for up to 6 h at room temperature under water and thus the APT does not need to be performed immediately after yeast collection. The correct AP value (maximum acidification produced by given yeast) is determined at 25 ± 0.1°C in a 15 mL sample containing ≥5% glucose and ≥1.5 g yeast wet weight. The cell concentration is conveniently measured as absorbance (A 660 ). Cell flocculation and/or sedimentation that can distort APT results can be prevented by stirring the sample at ≥200 rpm. The AP of yeast of different generations used to pitch brewery fermentations in cylindroconical tanks had a very low correlation with the wort half-attenuation time (T 1/2 ) due to large scatter, while each yeast generation separately showed a clear T 1/2 -AP relationship. The lowest AP of yeast cropped from cylindroconical tanks was displayed by the first cropped fraction. Post-cropping cooling had no effect on AP. Variations in pitching yeast vitality and their effect on the outcome of a brewery fermentation can be masked by variations in pitching rate, wort composition, ambient conditions in the cylindroconical tanks and other factors.
Exposure of beer to light results in the formation of undesirable flavours or complete spoilage. Photo-damaged beerhas a specific, so-called skunky or light-struck flavour (LSF). The compound responsible for LSF is 3-methylbut-2-ene-1-thiol (MBT). Riboflavin (RF) plays a key role in the formation of MBT. It absorbs light in the blue part of thespectrum and transfers excitation energy to isohumulones. This process is accompanied by the decomposition of RF,which causes a decrease in the absorbance of the sample at 450 nm. The decomposition is directly related to theformation of LSF. In this study, the decrease in absorbance associated with the defined illumination of model and realbeer directly in commercial bottles was measured. The decrease in absorbance correlated with the decrease in RFconcentration and the formation of LSF detected by the sensory panel. The Light-Struck Flavour Susceptibility Indexwas introduced as a rate of the beer susceptibility to light degradation and the formation of LSF.
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