>The aim of the existing work is to develop and validate a reversed-phase high-performance liquid chromatography (RP-HPLC) technique for the quantitative determination of methylcobalamin in bulk and t formulations that is easy, rapid, precise, accurate, affordable, and sensitive. Proceeding a princeton (C18) column with dimensions (250 x 4.6 mm, 5 μ), the isocratic elution technique was used. The mobile buffer phase comprised water (pH 6.5, adjusted with sodium chloride) and methanol in the relation (55:45) v/v. It was shown that the methylcobalamin retention time was 2.022 minutes under ideal circumstances. The correlation coefficient (r2) for the 900–2400 mcg/mL methylcobalamin concentration range, which was used to verify the method’s linearity, was 0.9994. Methylcobalamin had a recovery rate of 99.98–100.01% and RSD of 2%. The marketed t formulation test was successfully completed with 99.86%. The proposed and validated approach underwent the accuracy, precision, specificity, linearity, and system suitability testing advised by the ICH. RP-HPLC was used in the market formulation.
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