Legacy data from natural history collections contain invaluable and irreplaceable information about biodiversity in the recent past, providing a baseline for detecting change and forecasting the future of biodiversity on a human-dominated planet. However, these data are often not available in formats that facilitate use and synthesis. New approaches are needed to enhance the rates of digitization and data quality improvement. Notes from Nature provides one such novel approach by asking citizen scientists to help with transcription tasks. The initial web-based prototype of Notes from Nature is soon widely available and was developed collaboratively by biodiversity scientists, natural history collections staff, and experts in citizen science project development, programming and visualization. This project brings together digital images representing different types of biodiversity records including ledgers , herbarium sheets and pinned insects from multiple projects and natural history collections. Experts in developing web-based citizen science applications then designed and built a platform for transcribing textual data and metadata from these images. The end product is a fully open source web transcription tool built using the latest web technologies. The platform keeps volunteers engaged by initially explaining the scientific importance of the work via a short orientation, and then providing transcription “missions” of well defined scope, along with dynamic feedback, interactivity and rewards. Transcribed records, along with record-level and process metadata, are provided back to the institutions.  While the tool is being developed with new users in mind, it can serve a broad range of needs from novice to trained museum specialist. Notes from Nature has the potential to speed the rate of biodiversity data being made available to a broad community of users.

Aim We evaluated how an elevation gradient affects: (1) the availability of food required by a specialist seed-eater, Loxioides bailleui Oustalet (Drepanidinae), or palila, and hence the distribution of this endangered Hawaiian bird, and (2) the distribution of alien threats to Loxioides populations, their primary foods, and their dry-forest habitat, and hence strategies for their conservation.Location We worked throughout the subalpine forest that encircles Mauna Kea Volcano, Hawai'i Island, but we focused our studies mainly on the western slope between 2000 and 3000 m elevation, where the gradient of elevation was greatest and palila were most abundant.Methods We determined phenology and productivity patterns of the endemic dry-forest tree species, Sophora chrysophylla (Salisb.) Seem. (Fabaceae), or m a amane, which provides Loxioides with most of their food, and another common endemic tree, Myoporum sandwicense A. Gray (Myoporaceae), or naio, which provides some resources, along a 786-m elevation gradient at monthly intervals for 10 years (Sophora only). We also determined the availability each month of moth larvae (Lepidoptera) for that were important in the diet of nestling and adult palila. In addition, we documented the incidence of parasitism on moth larvae by several wasp (Hymenoptera) and fly (Diptera) species, and we determined the distribution of predatory wasps and ants (Hymenoptera), which potentially threaten insect prey of birds. Percentage cover of alien grass species that pose fire threats in palila habitat and other weeds were assessed during one survey. Small mammal abundance and distribution were determined by trapping during three (rodent) or five (carnivore) surveys.Results Sophora flower and seed (pod) availability varied predictably along the elevation gradient, with about 4 months separating peaks in reproduction at high and low elevations. This, together with highly variable production of flowers and pods within elevation strata, resulted in Sophora resources being available to Loxioides throughout the year on the western slope of Mauna Kea. Sophora produced flowers and pods more seasonally where gradients of elevation were short; thus, resources were available less consistently. In contrast, Myoporum produced flowers and fruits with little variation with respect to season or elevation. The availability of important insect prey of Loxioides was also related to elevation, in part because threats to Lepidoptera larvae from parasitic wasps were generally less at higher elevations. Threats to insect prey from predatory ants was also less at higher elevations but the abundance of predatory wasps was not related to elevation. Several weeds that pose the most serious threats to Loxioides habitat were more abundant at mid and low elevations, and alien grass cover was somewhat greater at mid elevation, thereby increasing fire risks in the

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New genetic diagnostic approaches have greatly aided efforts to document global biodiversity and improve biosecurity. This is especially true for organismal groups in which species diversity has been underestimated historically due to difficulties associated with sampling, the lack of clear morphological characteristics, and/or limited availability of taxonomic expertise. Among these methods, DNA sequence barcoding (also known as “DNA barcoding”) and by extension, meta‐barcoding for biological communities, has emerged as one of the most frequently utilized methods for DNA‐based species identifications. Unfortunately, the use of DNA barcoding is limited by the availability of complete reference libraries (i.e., a collection of DNA sequences from morphologically identified species), and by the fact that the vast majority of species do not have sequences present in reference databases. Such conditions are critical especially in tropical locations that are simultaneously biodiversity rich and suffer from a lack of exploration and DNA characterization by trained taxonomic specialists. To facilitate efforts to document biodiversity in regions lacking complete reference libraries, we developed a novel statistical approach that categorizes unidentified species as being either likely native or likely nonnative based solely on measures of nucleotide diversity. We demonstrate the utility of this approach by categorizing a large sample of specimens of terrestrial insects and spiders (collected as part of the Moorea BioCode project) using a generalized linear mixed model (GLMM). Using a training data set of known endemic (n = 45) and known introduced species (n = 102), we then estimated the likely native/nonnative status for 4,663 specimens representing an estimated 1,288 species (412 identified species), including both those specimens that were either unidentified or whose endemic/introduced status was uncertain. Using this approach, we were able to increase the number of categorized specimens by a factor of 4.4 (from 794 to 3,497), and the number of categorized species by a factor of 4.8 from (147 to 707) at a rate much greater than chance (77.6% accuracy). The study identifies phylogenetic signatures of both native and nonnative species and suggests several practical applications for this approach including monitoring biodiversity and facilitating biosecurity.

Oboyski, Peter T.; Slotterback, John W.; and Banko, Paul C., "Differential parasitism of seed-feeding Cydia (Lepidoptera: Tortricidae) by native and alien wasp species relative to elevation in subalpine Sophora (Fabaceae) forests on Mauna Kea, Hawaii" (2004 Key words: Alien species, Biological control, Elevation gradient, Moth conservation, Non-target, Parasitism AbstractAlien parasitic wasps, including accidental introductions and purposefully released biological control agents, have been implicated in the decline of native Hawaiian Lepidoptera. Understanding the potential impacts of alien wasps requires knowledge of ecological parameters that influence parasitism rates for species in their new environment. Sophora seed-feeding Cydia spp. (Lepidoptera: Tortricidae) were surveyed for larval parasitoids to determine how native and alien wasps are partitioned over an elevation gradient (2200-2800 m) on Hawaii Island, Hawaii. Parasitism rate of native Euderus metallicus (Eulophidae) increased with increased elevation, while parasitism rate by immigrant Calliephialtes grapholithae (Ichneumonidae) decreased. Parasitism by Pristomerus hawaiiensis (Ichneumonidae), origins uncertain, also decreased with increased elevation. Two other species, Diadegma blackburni (Ichneumonidae), origins uncertain, and Brasema cushmani (Eupelmidae), a purposefully introduced biological control agent for pepper weevil, did not vary significantly with elevation. Results are contrasted with a previous study of this system with implications for the conservation of an endangered bird species that feed on Cydia larvae. Interpretation of results is hindered by lack of knowledge of autecology of moths and wasps, origins, phylogeny, systematics, competitive ability, and physiological limitations of each wasp species. These factors should be incorporated into risk analysis for biological control introductions and invasive species programs.

Abstract. The emergence rate of new plant diseases is increasing due to novel introductions, climate change, and changes in vector populations, posing risks to agricultural sustainability. Assessing and managing future disease risks depends on understanding the causes of contemporary and historical emergence events. Since the mid-1990s, potato growers in the western United States, Mexico, and Central America have experienced severe yield loss from Zebra Chip disease and have responded by increasing insecticide use to suppress populations of the insect vector, the potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae). Despite the severe nature of Zebra Chip outbreaks, the causes of emergence remain unknown. We tested the hypotheses that (1) B. cockerelli occupancy has increased over the last century in California and (2) such increases are related to climate change, specifically warmer winters. We compiled a data set of 87,000 museum specimen occurrence records across the order Hemiptera collected between 1900 and 2014. We then analyzed changes in B. cockerelli distribution using a hierarchical occupancy model using changes in background species lists to correct for collecting effort. We found evidence that B. cockerelli occupancy has increased over the last century. However, these changes appear to be unrelated to climate changes, at least at the scale of our analysis. To the extent that species occupancy is related to abundance, our analysis provides the first quantitative support for the hypothesis that B. cockerelli population abundance has increased, but further work is needed to link B. cockerelli population dynamics to Zebra Chip epidemics. Finally, we demonstrate how this historical macro-ecological approach provides a general framework for comparative risk assessment of future pest and insect vector outbreaks.

The digitization of biocollections is a critical task with direct implications for the global community who use the data for research and education. Recent innovations to involve citizen scientists in digitization increase awareness of the value of biodiversity specimens; advance science, technology, engineering, and math literacy; and build sustainability for digitization. In support of these activities, we launched the first global citizen-science event focused on the digitization of biodiversity specimens: Worldwide Engagement for Digitizing Biocollections (WeDigBio). During the inaugural 2015 event, 21 sites hosted events where citizen scientists transcribed specimen labels via online platforms (DigiVol, Les Herbonautes, Notes from Nature, the Smithsonian Institution's Transcription Center, and Symbiota). Many citizen scientists also contributed off-site. In total, thousands of citizen scientists around the world completed over 50,000 transcription tasks. Here, we present the process of organizing an international citizen-science event, an analysis of the event's effectiveness, and future directions—content now foundational to the growing WeDigBio event.

The recently completed Odonata database for California consists of specimen records from the major entomology collections of the state, large Odonata collections outside of the state, previous literature, historical and recent field surveys, and from enthusiast group observations. The database includes 32,025 total records and 19,000 unique records for 106 species of dragonflies and damselflies, with records spanning 1879–2013. Records have been geographically referenced using the point-radius method to assign coordinates and an uncertainty radius to specimen locations. In addition to describing techniques used in data acquisition, georeferencing, and quality control, we present assessments of the temporal, spatial, and taxonomic distribution of records. We use this information to identify biases in the data, and to determine changes in species prevalence, latitudinal ranges, and elevation ranges when comparing records before 1976 and after 1979. The average latitude of where records occurred increased by 78 km over these time periods. While average elevation did not change significantly, the average minimum elevation across species declined by 108 m. Odonata distribution may be generally shifting northwards as temperature warms and to lower minimum elevations in response to increased summer water availability in low-elevation agricultural regions. The unexpected decline in elevation may also be partially the result of bias in recent collections towards centers of human population, which tend to occur at lower elevations. This study emphasizes the need to address temporal, spatial, and taxonomic biases in museum and observational records in order to produce reliable conclusions from such data.