No abstract

A new method for fabrication of metal crowns has been developed by one of the authors (M.A.). There are two principles involved: machine duplication of models and electric discharge machining. The metal used is pure unalloyed titanium, which is processed as a coping and later covered by a composite resin. In 1986, 205 separate titanium crowns were made on 149 patients. One year later 192 crowns on 137 patients could be examined. Five crowns had been replaced by new ones owing to fracture of the composite resin. In accordance with the CDA quality evaluation system the following results were obtained for the remaining 187 crowns: margin integrity, 186 excellent or satisfactory (99.5%); anatomic form, 185 excellent or satisfactory (98.9%); and surface and color, 181 excellent or satisfactory results (96.8%). The 1-year results are promising, and further follow-up studies will be made.

In 1986, 149 patients were provided with titanium crowns. The method used for fabrication of the titanium copings involved two principles: machine duplication of models and electric discharge machining. For the veneering of the copings, Isosit was used for the first 90% of the crowns in the series, and the Dentacolor-Silicoater technique for the last 10%. Of 205 individual crowns cemented in 1986, 167 could be examined after 2 years. The crowns were rated by four independent examiners using the CDA quality evaluation system. Bleeding Index and Margin Index were also used. The Margin Integrity score was recorded as satisfactory for all crowns examined over the period studied. A vast majority of the margins were rated as excellent. Isosit (n = 145) disclosed shortcomings including fractures and substantial deteriorations of Surface and Color and of Anatomic Form. With Dentacolor as veneering material (n = 18) the results with the factors Surface and Color and Anatomic Form were still rated satisfactory after 2 years, and no fractures of the veneering material were registered. Bleeding Index and Margin Index showed comparatively small changes after 2 years.

We have investigated the presence and form of glutamine synthetase (GS) in Frankia vesicle cluster preparations of two actively nitrogen‐fixing Frankia‐Alnus incana root‐nodule symbioses and in cultured Frankia sp. strain CpI1 (HFP070101). The symbioses contained Frankia CpI1 or the local source of Frankia. We used Western‐blot analysis with antisera raised against three types of GS. In symbiotic Frankia GS protein was not detected at a significant level when either antisera against Rhodospirillum rubrum GS or antisera against Rhizobium meliloti GSII were used. In cultured Frankia CpI1 GSI was detected both when grown with NH4+ or N2 as nitrogen source, and GSII was detected when grown on N2. Antiserum raised against the nodule‐specific GSn1 of Phaseolus vulgaris crossreacted with a 43‐kDa polypeptide corresponding to plant GS in root‐nodule extracts from Alnus, and with a 41‐kDa polypeptide corresponding to GSII in cultured Frankia CpI1 grown on N2. We conclude that both GSI and GSII are repressed in symbiotic Frankia and that NH4+ produced through nitrogen fixation is assimilated by the plant in Frankia‐Alnus incana symbioses. It thus appears that vesicle formation, synthesis of nitrogenase and synthesis of GS are separately regulated in symbiotic Frankia and that the plant has to supply symbiotic Frankia with organic nitrogen in some form in addition to the carbon.