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2000 amines amines (acyclic compounds) P 0060 09 -078 Synthesis of New 2,3-Perfluoroalkyl-and Perfluoroaryl-1,4diazabutadienes (α-Diimines) -The new title compounds (III) and (V) are useful precursors of metallodiimines and heterocycles. -(DIEL, BRUCE N.; DEARDORFF, PETER J.; ZELENSKI, CATHERINE M.; Tetrahedron Lett. 40 (1999) 49, 8523-8527; Dep. Chem. Sci., Midwest Res. Inst., Kansas City, MO 64110, USA; EN)

Methylseleno-L-cysteine (L-SeMC) is a naturally occurring amino acid analogue used as a general dietary supplement and is being explored as a chemopreventive agent. As a known dietary supplement, L-SeMC is not regulated as a pharmaceutical and there is a paucity of analytical methods available. To address the lack of methodology, a stability-indicating method was developed and validated to evaluate L-SeMC as both the bulk drug and formulated drug product (400 µg Se/capsule). The analytical approach presented is a simple, nonderivatization method that utilizes HPLC with ultraviolet detection at 220 nm. A C18 column with a volatile ion-pair agent and methanol mobile phase was used for the separation. The method accuracy was 99-100% from 0.05 to 0.15 mg/mL L-SeMC for the bulk drug, and 98-99% from 0.075 to 0.15 mg/mL L-SeMC for the drug product. Method precision was <1% for the bulk drug and was 3% for the drug product. The LOQ was 0.1 µg/mL L-SeMC or 0.002 µg L-SeMC on column.

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