Objectives Intra-articular injections of local anaesthetics (LA), glucocorticoids (GC), or hyaluronic acid (HA) are used to treat osteoarthritis (OA). Contrast agents (CA) are needed to prove successful intra-articular injection or aspiration, or to visualize articular structures dynamically during fluoroscopy. Tranexamic acid (TA) is used to control haemostasis and prevent excessive intra-articular bleeding. Despite their common usage, little is known about the cytotoxicity of common drugs injected into joints. Thus, the aim of our study was to investigate the effects of LA, GC, HA, CA, and TA on the viability of primary human chondrocytes and tenocytes in vitro . Methods Human chondrocytes and tenocytes were cultured in a medium with three different drug dilutions (1:2; 1:10; 1:100). The following drugs were used to investigate cytotoxicity: lidocaine hydrochloride 1%; bupivacaine 0.5%; triamcinolone acetonide; dexamethasone 21-palmitate; TA; iodine contrast media; HA; and distilled water. Normal saline served as a control. After an incubation period of 24 hours, cell numbers and morphology were assessed. Results Using LA or GC, especially triamcinolone acetonide, a dilution of 1:100 resulted in only a moderate reduction of viability, while a dilution of 1:10 showed significantly fewer cell counts. TA and CA reduced viability significantly at a dilution of 1:2. Higher dilutions did not affect viability. Notably, HA showed no effects of cytotoxicity in all drug dilutions. Conclusion The toxicity of common intra-articular injectable drugs, assessed by cell viability, is mainly dependent on the dilution of the drug being tested. LA are particularly toxic, whereas HA did not affect cell viability. Cite this article : P. Busse, C. Vater, M. Stiehler, J. Nowotny, P. Kasten, H. Bretschneider, S. B. Goodman, M. Gelinsky, S. Zwingenberger. Cytotoxicity of drugs injected into joints in orthopaedics. Bone Joint Res 2019;8:41–48. DOI: 10.1302/2046-3758.82.BJR-2018-0099.R1.
An important aspect of the aerodynamic flow field in the tip region of axial compressor rotors is the unsteady interaction between the tip clearance vortex (TCV) and the incoming stator wakes. In order to gain an improved understanding of the mechanics involved, systematic studies need to be performed. As a first step toward the characterization of the dynamic effects caused by the relative movement of the blade rows, the impact of a stationary wake-induced inlet disturbance on a linear compressor cascade with tip clearance will be analyzed. The wakes were generated by a fixed grid of cylindrical bars with variable pitch being placed at discrete pitchwise positions. This paper focuses on experimental studies conducted at the newly designed low-speed cascade wind tunnel in Dresden. The general tunnel configuration and details on the specific cascade setup will be presented. Steady state flow field measurements were carried out using five-hole probe traverses up- and downstream of the cascade and accompanied by static wall pressure readings. 2D-particle image velocimetry (PIV) measurements complemented these results by visualizing the blade-to-blade flow field. Hence, the structure of the evolving secondary flow system is evaluated and compared for all tested configurations.
An important aspect of the aerodynamic flow field in the tip region of axial compressor rotors is the unsteady interaction between the tip clearance vortex and the incoming stator wakes. In order to gain an improved understanding of the mechanics involved, systematic studies need to be performed. As a first step towards the characterisation of the dynamic effects caused by the relative movement of the blade rows, the impact of a stationary wake-induced inlet disturbance on a linear compressor cascade with tip clearance will be analyzed. The wakes were generated by a fixed grid of cylindrical bars with variable pitch being placed at discrete pitchwise positions. Part II of this two-part paper focuses on the numerical studies conducted with the scientific flow solver TRACE. Selected measurements, which are discussed in detail in the first part of this paper, are compared with steady state RANS simulation data to determine the validity of the computational model. For this purpose, the flow field obtained in the passage (PIV), at the cascade exit (five-hole probes) and the endwall pressure distributions were used. The presented numerical results show potentials and limitations of the steady state CFD for the prediction of the investigated flow phenomena. The computations provide the initial conditions for future unsteady calculations, and enable a separate depiction of potential effects of steady and unsteady wake-tip clearance vortex interaction.
An important aspect of the aerodynamic flow field in the tip region of axial compressor rotors is the unsteady interaction between the tip clearance vortex and the incoming stator wakes. In order to gain an improved understanding of the mechanics involved, systematic studies need to be performed. As a first step towards the characterisation of the dynamic effects caused by the relative movement of the blade rows, the impact of a stationary wake-induced inlet disturbance on a linear compressor cascade with tip clearance will be analysed. The wakes were generated by a fixed grid of cylindrical bars with variable pitch being placed at discrete pitchwise positions. Part I of this two-part paper focuses on experimental studies conducted at the newly designed low-speed cascade wind tunnel in Dresden. The general tunnel configuration and details on the specific cascade setup will be presented. Steady state flow field measurements were carried out using five-hole probe traverses up- and downstream of the cascade and accompanied by static wall pressure readings. 2D-PIV measurements complemented these results by visualizing the blade-to-blade flow field. Hence, the structure of the evolving secondary flow system is evaluated and compared for all tested configurations.
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